光学分子成像监测IRDye 800CW和gx1 -共轭聚乳酸纳米颗粒包封恩度对胶质瘤的肿瘤靶向和治疗效果。

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Molecular Imaging Pub Date : 2015-01-01
Yaqian Li, Yang Du, Xia Liu, Qian Zhang, Lijia Jing, Xiaolong Liang, Chongwei Chi, Zhifei Dai, Jie Tian
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引用次数: 0

摘要

分子影像学在肿瘤诊断和治疗反应监测中的应用在胶质母细胞瘤(GBM)研究中具有重要意义。抗血管生成治疗是目前GBM治疗的新兴方法之一。本研究制备了一种多功能纳米颗粒,可以促进肿瘤的荧光成像,并在体内将治疗剂输送到肿瘤区域,因此在癌症的诊断和治疗中具有广泛的应用。该颗粒是包封恩度的聚乳酸(PLA)纳米颗粒,进一步与GX1肽和近红外(NIR)染料IRDye 800CW (IGPNE)结合。我们利用包括近红外荧光分子成像(FMI)和生物发光成像(BLI)在内的双模光学分子成像技术,在体内证明了IGPNE对U87MG异种移植物的无创血管生成靶向和治疗。近红外FMI结果表明,与游离的IRDye 800CW相比,IGPNE在肿瘤部位有更多的积累。为进一步评价IGPNE的抗肿瘤作用,对荷瘤小鼠进行BLI和免疫组化分析。在分子影像学的辅助下,结果证实IGPNE与游离的恩度相比,抗肿瘤治疗效果增强。综上所述,IGPNE实现了U87MG肿瘤的实时成像,提高了体内抗血管生成的治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Monitoring Tumor Targeting and Treatment Effects of IRDye 800CW and GX1-Conjugated Polylactic Acid Nanoparticles Encapsulating Endostar on Glioma by Optical Molecular Imaging.

Molecular imaging used in cancer diagnosis and therapeutic response monitoring is important for glioblastoma (GBM) research. Antiangiogenic therapy currently is one of the emerging approaches for GBM treatment. In this study, a multifunctional nanoparticle was fabricated that can facilitate the fluorescence imaging of tumor and deliver a therapeutic agent to the tumor region in vivo and therefore possesses broad application in cancer diagnosis and treatment. This particle was polylactic acid (PLA) nanoparticles encapsulating Endostar, which was further conjugated with GX1 peptide and the near-infrared (NIR) dye IRDye 800CW (IGPNE). We demonstrated noninvasive angiogenesis targeting and therapy of IGPNE on U87MG xenografts in vivo using dual-modality optical molecular imaging including NIR fluorescence molecular imaging (FMI) and bioluminescence imaging (BLI). The NIR FMI results demonstrated that IGPNE had more accumulation to the tumor site compared to free IRDye 800CW. To further evaluate the antitumor treatment efficacy of IGPNE, BLI and immunohistochemistry analysis were performed on tumor-bearing mice. With the aid of molecular imaging, the results confirmed that IGPNE enhanced antitumor treatment efficacy compared to free Endostar. In conclusion, IGPNE realizes real-time imaging of U87MG tumors and improves the antiangiogenic therapeutic efficacy in vivo.

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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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