雌激素对大鼠睾丸偏心运动损伤的影响。

IF 0.2 4区 医学
Iranian Red Crescent Medical Journal Pub Date : 2015-04-25 eCollection Date: 2015-04-01 DOI:10.5812/ircmj.17(4)2015.22521
Serpil Can, Jale Selli, Basak Buyuk, Sergulen Aydin, Ramazan Kocaaslan, Gulname Findik Guvendi
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引用次数: 1

摘要

背景:近年来,人们对炎症反应和氧化应激与组织损伤和运动疲劳相关的可能机制进行了大量的科学研究。众所周知,自由基可以在体外条件下诱导,也可以通过穷尽性体育锻炼引起氧化应激。目的:采用组织学和体视学方法,探讨运动联合使用合成代谢类固醇对睾丸精子发生过程的影响。材料与方法:将36只雄性Sprague Dawley大鼠分为6组,分别为对照组、偏心运动组、施雌激素组、偏心运动后1小时施雌激素并解剖组、偏心运动后48小时不施雌激素并解剖组和偏心运动后48小时施雌激素并解剖组。在啮齿类动物电动跑步机上进行偏心运动,应用雌激素组每日皮下注射生理剂量。采用特定的组织病理学、免疫组织化学和体视学方法检查睾丸组织。采用TUNEL法对睾丸组织切片进行染色,鉴定凋亡细胞。细胞凋亡以阳性细胞百分比计算,采用立体分析。采用单因素方差分析(ANOVA)对立体分析所得数据进行统计分析。结果:常规光镜检查显示,偏心运动组和雌激素补充组睾丸组织均出现轻微损伤。在同时进行怪癖运动和补充雌激素的组中,睾丸组织的恶化程度更高。同样,免疫组织化学结果在反常运动组和雌激素补充组中也更为突出。结论:雌激素的补充增加了偏心运动对睾丸组织的损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effect of estrogen usage on eccentric exercise-induced damage in rat testes.

The effect of estrogen usage on eccentric exercise-induced damage in rat testes.

The effect of estrogen usage on eccentric exercise-induced damage in rat testes.

The effect of estrogen usage on eccentric exercise-induced damage in rat testes.

Background: Recent years, lots of scientific studies are focused on the possible mechanism of inflammatory response and oxidative stress which are the mechanism related with tissue damage and exercise fatigue. It is well-known that free oxygen radicals may be induced under invitro conditions as well as oxidative stress by exhaustive physical exercise.

Objectives: The aim of this study was to investigate the effects of anabolic steroids in conjunction with exercise in the process of spermatogenesis in the testes, using histological and stereological methods.

Materials and methods: Thirty-six male Sprague Dawley rats were divided to six groups, including the control group, the eccentric exercise administered group, the estrogen applied group, the estrogen applied and dissected one hour after eccentric exercise group, the no estrogen applied and dissected 48 hours after eccentric exercise group and the estrogen applied and dissected 48 hours after eccentric exercise group. Eccentric exercise was performed on a motorized rodent treadmill and the estrogen applied groups received daily physiological doses by subcutaneous injections. Testicular tissues were examined using specific histopathological, immunohistochemical and stereological methods. Sections of the testes tissue were stained using the TUNEL method to identify apoptotic cells. Apoptosis was calculated as the percentage of positive cells, using stereological analysis. A statistical analysis of the data was carried out with one-way analysis of variance (ANOVA) for the data obtained from stereological analysis.

Results: Conventional light microscopic results revealed that testes tissues of the eccentric exercise administered group and the estrogen supplemented group exhibited slight impairment. In groups that were both eccentrically exercised and estrogen supplemented, more deterioration was detected in testes tissues. Likewise, immunohistochemistry findings were also more prominent in the eccentrically exercised and estrogen supplemented groups.

Conclusions: The findings suggest that estrogen supplementation increases damage in testicular tissue due to eccentric exercise.

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来源期刊
Iranian Red Crescent Medical Journal
Iranian Red Crescent Medical Journal 医学-医学:内科
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期刊介绍: The IRANIAN RED CRESCENT MEDICAL JOURNAL is an international, English language, peer-reviewed journal dealing with general Medicine and Surgery, Disaster Medicine and Health Policy. It is an official Journal of the Iranian Hospital Dubai and is published monthly. The Iranian Red Crescent Medical Journal aims at publishing the high quality materials, both clinical and scientific, on all aspects of Medicine and Surgery
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