新兴的光学纳米技术。

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2015-09-29 eCollection Date: 2015-01-01 DOI:10.2147/NSA.S50042
Paul C Montgomery, Audrey Leong-Hoi
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引用次数: 15

摘要

为了面对现代医疗保健的挑战,需要具有亚细胞分辨率或宽视场检测的新成像技术。远场光学纳米技术提供了许多新的解决方案,提供高分辨率或高速检测。我们提出了一个新的分类方案,以帮助了解越来越多的光学纳米技术。我们强调了超分辨率技术和纳米检测技术之间的重要区别,超分辨率技术提供了改进的分辨率和纳米检测技术来表征未解析的纳米结构。这两类中的一些新兴技术在生物物理学和医学方面的应用得到了强调。最新技术采用数字全息和散射透镜显微镜的广角成像技术,可以实现亚细胞甚至体内的超分辨率成像,而无需标记。纳米检测技术分为四个子类别,使用对比,相位,反褶积和纳米标记。对比增强是通过基于偏振光的技术和用频闪灯相衬显微镜来揭示纳米结构。非常高的灵敏度相位测量使用干涉显微镜显示提供纳米表面粗糙度测量或揭示内部纳米结构。最后,纳米标记的使用是用随机荧光显微镜来绘制细胞内结构。我们还提出了光学纳米技术的一些未来前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Emerging optical nanoscopy techniques.

Emerging optical nanoscopy techniques.

Emerging optical nanoscopy techniques.

Emerging optical nanoscopy techniques.

To face the challenges of modern health care, new imaging techniques with subcellular resolution or detection over wide fields are required. Far field optical nanoscopy presents many new solutions, providing high resolution or detection at high speed. We present a new classification scheme to help appreciate the growing number of optical nanoscopy techniques. We underline an important distinction between superresolution techniques that provide improved resolving power and nanodetection techniques for characterizing unresolved nanostructures. Some of the emerging techniques within these two categories are highlighted with applications in biophysics and medicine. Recent techniques employing wider angle imaging by digital holography and scattering lens microscopy allow superresolution to be achieved for subcellular and even in vivo, imaging without labeling. Nanodetection techniques are divided into four subcategories using contrast, phase, deconvolution, and nanomarkers. Contrast enhancement is illustrated by means of a polarized light-based technique and with strobed phase-contrast microscopy to reveal nanostructures. Very high sensitivity phase measurement using interference microscopy is shown to provide nanometric surface roughness measurement or to reveal internal nanometric structures. Finally, the use of nanomarkers is illustrated with stochastic fluorescence microscopy for mapping intracellular structures. We also present some of the future perspectives of optical nanoscopy.

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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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