维生素E和褪黑素对睡眠剥夺Wistar大鼠血清睾酮水平的影响。

O O Akindele, O T Kunle-Alabi, D H Adeyemi, B O Oghenetega, Y Raji
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引用次数: 0

摘要

背景:睡眠剥夺影响着全球很大一部分人口。据报道,它可以诱导睾丸氧化应激并降低血清睾酮水平。众所周知,外源性抗氧化剂可以预防与氧化应激相关的损害和疾病,但对其在睡眠剥夺期间的有效性知之甚少。目的:研究两种抗氧化剂的抗氧化作用;褪黑素和维生素E对睡眠剥夺雄性Wistar大鼠血清睾酮浓度的影响。方法:雄性Wistar大鼠30只。动物分为6组(n = 5),第1组为对照组,第2组为剥夺睡眠组,第3组仅给予维生素E (200 mg/kg bwt),第4组给予维生素E并剥夺睡眠组,第5组仅给予褪黑素(10 mg/kg bwt),第6组给予褪黑素(10 mg/kg bwt)并剥夺睡眠组。采用改进的多平台技术诱导睡眠剥夺。分别于第7、14、21天测定体重。在献祭时采集血液,提取血清用于睾酮、皮质酮和褪黑激素的分析。睾丸丙二醛、超氧化物歧化酶和过氧化氢酶水平分别采用Adam-Vizi和Seregi(1982)、Misra和Fridovich(1972)和Sinha(1972)的方法测定。所得资料采用单因素方差分析,p < 0.05为显著性。结果:与对照组(3.3±0.04)、缺觉+维生素E组(2.8±0.5)、缺觉+褪黑素组(2.0±0.3)相比,缺觉动物血清睾酮(0.6±0.3)显著降低(p < 0.05)。与对照组相比,褪黑激素+睡眠不足组的睾丸激素水平降低。与睡眠不足组相比,各组血清皮质酮(nmol/l)和褪黑激素水平均无显著变化。睡眠剥夺组睾丸丙二醛(MDA)(1.6±0.1单位/mg)、超氧化物歧化酶(SOD)(3.2±0.2单位/mg)、过氧化氢醇(44.3±1.1单位/mg)水平均高于对照组(0.9±0.0 μmg)。与缺觉组相比,缺觉组和维生素E组MDA和过氧化氢酶显著降低(1.1±0.2、2.4±0.3、39±1.0单位/ mg),而单独褪黑素组MDA升高。水平(1.7±0.2单位/毫克)与对照组比较。睡眠剥夺+褪黑素组SOD(2.7±0.2 μ/mg)较对照组升高(p < 0.05),而MDA和过氧化氢酶水平与睡眠剥夺组比较无显著差异。结论:维生素E在维持睡眠不足Wistar大鼠睾丸激素水平方面的作用比褪黑激素更有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of vitamin E and melatonin on serum testosterone level in sleep deprived Wistar rats.

Background: Sleep deprivation affects a significant proportion of the global population. It has been reported to induce oxidative stress in the testes and reduce serum testosterone levels. Exogenous anti-oxidants have been known to prevent damages and diseases associated with oxidative stress but there is dearth of knowledge on their effectiveness during sleep deprivation.

Aim: This study was designed to investigate the effects of two anti-oxidants; melatonin and vitamin E on serum testosterone concentration in sleep deprived male Wistar rats.

Methods: Thirty (30) male Wistar rats were used for this study. Animals were divided into six (6) groups (n = 5). Group 1 was the control, group 2 rats were sleep deprived, group 3 received vitamin E (200 mg/ kg bwt) only, group 4 rats received vitamin E and were sleep deprived, group 5 received melatonin only (10 mg/kg bwt), and group 6 rats received melatonin (10 mg/kg bwt) and were sleep deprived. Sleep deprivation was induced using the modified multiple platform technique. Body weights were taken on days 7, 14 and 21. Blood was collected at sacrifice and serum was obtained for analyses of testosterone, corticosterone and melatonin. Testicular malondialdehyde, superoxide dismutase and catalase levels were determined by the methods of Adam-Vizi and Seregi (1982), Misra and Fridovich (1972), and Sinha, (1972) respectively. Data obtained were analyzed using one way ANOVA and p < 0.05 was considered significant.

Results: Serum testosterone (nmol/l) of the sleep deprived animals (0.6 ± 0.3) reduced significantly (p < 0.05) compared with control group (3.3 ± 0.04), sleep deprived+vitamin E group (2.8 ± 0.5) and sleep deprived+melatonin group (2.0 ± 0.3). Also, melatonin+sleep deprived group had reduced testosterone compared with control. There were no significant changes in the serum corticosterone (nmol/l) and melatonin levels in all the groups compared with the sleep deprived group. However, corticosterone was increased in the sleep deprived+Vitamin E group (51.6 ± 20.5) compared with control (6.3 ± 0.6) Sleep deprived group had increased testicular malondialdehyde (MDA) (1.6 ± 0.1 unit/mg), superoxide dismutase (SOD) (3.2 ± 0.2 unit/mg), and catalasel evels (44.3 ± 1.1 unit/ mg) compared with control (0.9 ± 0.0 μmg). MDA, and catalase were significantly reduced in sleep deprived+vitamin E (1.1 ± 0.2, 2.4 ± 0.3, 39 ± 1.0 unit/ mg) compared with sleep deprived while melatonin alone had increased MDA. level (1.7 ± 0.2unit/mg) compared with control. SOD in the sleep deprived+melatonin group (2.7 ± 0.2 μ/mg) as compared with control increased (p < 0.05) while MDA and catalase levels as compared with control and sleep deprived groups showed no difference. Histological findings showed that the pathology in the testes of sleep deprived rats was ameliorated by vitamin E.

Conclusion: Vitamin E had a more potent effect than melatonin in maintaining testosterone level in sleep deprived Wistar rat.

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