{"title":"调查孟加拉国南部新生儿和青少年克雷伯菌的抗菌素耐药性和产生ESBL的基因","authors":"Afroza Akter Tanni, Nahid Sultana, Wazir Ahmed, Md Mahbub Hasan, Md Shakhawat Hossain, Sajjad Hossain Noyon, Md Mobarok Hossain, Adnan Mannan","doi":"10.1155/2022/7071009","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Multidrug-resistant (MDR) clones of <i>Klebsiella pneumoniae</i> (<i>Kpn</i>) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum <i>β</i>-lactamases (ESBLs) are a rapidly evolving group of <i>β</i>-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of <i>Kpn</i> antibiotic resistance in different areas of the southern part of Bangladesh.</p><p><strong>Methods: </strong>This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect <i>Kpn</i>'s sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene bla<sub>SHV-11</sub> was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the bla<sub>SHV-11</sub> gene.</p><p><strong>Results: </strong>Almost all of the <i>Kpn</i> isolates were spotted to be antibiotic-resistant. These <i>Kpn</i> isolates were resistant to <i>β</i>-lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving <i>Kpn</i> were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels (<i>p</i> < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene bla<sub>SHV-11</sub> was found in 38% isolates.</p><p><strong>Conclusion: </strong>The significant frequency of MDR <i>Kpn</i> harboring <i>β</i>-lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553706/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigating Antimicrobial Resistance and ESBL Producing Gene in <i>Klebsiella</i> Isolates among Neonates and Adolescents in Southern Bangladesh.\",\"authors\":\"Afroza Akter Tanni, Nahid Sultana, Wazir Ahmed, Md Mahbub Hasan, Md Shakhawat Hossain, Sajjad Hossain Noyon, Md Mobarok Hossain, Adnan Mannan\",\"doi\":\"10.1155/2022/7071009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Multidrug-resistant (MDR) clones of <i>Klebsiella pneumoniae</i> (<i>Kpn</i>) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum <i>β</i>-lactamases (ESBLs) are a rapidly evolving group of <i>β</i>-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of <i>Kpn</i> antibiotic resistance in different areas of the southern part of Bangladesh.</p><p><strong>Methods: </strong>This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect <i>Kpn</i>'s sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene bla<sub>SHV-11</sub> was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the bla<sub>SHV-11</sub> gene.</p><p><strong>Results: </strong>Almost all of the <i>Kpn</i> isolates were spotted to be antibiotic-resistant. These <i>Kpn</i> isolates were resistant to <i>β</i>-lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving <i>Kpn</i> were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels (<i>p</i> < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene bla<sub>SHV-11</sub> was found in 38% isolates.</p><p><strong>Conclusion: </strong>The significant frequency of MDR <i>Kpn</i> harboring <i>β</i>-lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.</p>\",\"PeriodicalId\":50715,\"journal\":{\"name\":\"Canadian Journal of Infectious Diseases & Medical Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2022-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553706/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian Journal of Infectious Diseases & Medical Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1155/2022/7071009\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Infectious Diseases & Medical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2022/7071009","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Investigating Antimicrobial Resistance and ESBL Producing Gene in Klebsiella Isolates among Neonates and Adolescents in Southern Bangladesh.
Background: Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum β-lactamases (ESBLs) are a rapidly evolving group of β-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of Kpn antibiotic resistance in different areas of the southern part of Bangladesh.
Methods: This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect Kpn's sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene blaSHV-11 was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the blaSHV-11 gene.
Results: Almost all of the Kpn isolates were spotted to be antibiotic-resistant. These Kpn isolates were resistant to β-lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving Kpn were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels (p < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene blaSHV-11 was found in 38% isolates.
Conclusion: The significant frequency of MDR Kpn harboring β-lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.
期刊介绍:
Canadian Journal of Infectious Diseases and Medical Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to infectious diseases of bacterial, viral and parasitic origin. The journal welcomes articles describing research on pathogenesis, epidemiology of infection, diagnosis and treatment, antibiotics and resistance, and immunology.