四氯化苦通过抑制 TRPC6 介导的 RhoA/ROCK1 通路减轻荚膜细胞损伤

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Analytical Cellular Pathology Pub Date : 2022-09-30 eCollection Date: 2022-01-01 DOI:10.1155/2022/7534181
Lichan Mao, Yin Ding, Dongrong Yu, Jiazhen Yin, Jin Yu
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引用次数: 0

摘要

四氢嘌呤(Tet)是一种存在于传统中药中的化合物,具有保护肾功能的作用。我们的研究旨在阐明 Tet 对荚膜细胞损伤的疗效和内在机制。本研究用阿霉素(ADR)诱导大鼠荚膜细胞损伤,并构建了 TRPC6 过表达的 MPC5 荚膜细胞。我们发现,Tet 治疗降低了 ADR 诱导的大鼠的蛋白尿、血清肌酐和血尿素氮水平,并提高了血浆白蛋白水平。Tet减少了细胞内Ca2+的流入,并减少了过表达TRPC6的MPC5荚膜细胞的凋亡。Tet 下调了肾脏 TRPC6、RhoA 和 ROCK1 的表达,并上调了突触素的表达;同时,它还降低了体内和体外钙神经蛋白的活性。总之,Tet通过影响TRPC6及其下游的RhoA/ROCK1信号通路来保护荚膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway.

Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway.

Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway.

Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway.

Tetrandrine (Tet), a compound found in a traditional Chinese medicine, presents the protective effect for kidney function. Our study is aimed at clarifying the efficacy and underlying mechanism of Tet on podocyte injury. In this study, podocyte injury was induced in rats with adriamycin (ADR), and MPC5 podocytes were constructed with TRPC6 overexpression. We found that Tet treatment reduced the levels of proteinuria, serum creatinine, and blood urea nitrogen and increased plasma albumin levels in ADR-induced rats. Tet reduced intracellular Ca2+ influx and apoptosis in MPC5 podocytes overexpressing TRPC6. Tet downregulated the expression of renal TRPC6, RhoA, and ROCK1 and upregulated the expression of synaptopodin; meanwhile, it reduced calcineurin activity in vivo and in vitro. In conclusion, Tet protects against podocyte by affecting TRPC6 and its downstream RhoA/ROCK1 signaling pathway.

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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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