利用毛细管区带电泳分析细胞培养基样品中的阳离子维生素。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-10-06 eCollection Date: 2022-01-01 DOI:10.1155/2022/2819855

Debbie van der Burg, Hermann Wätzig, Cari E Sänger-van de Griend

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引用次数: 0

摘要

本文介绍了一种毛细管电泳法，用于测定未经处理的细胞培养基样品中的阳离子B族维生素硫胺素、烟酰胺、吡哆醇、吡哆醛和吡哆胺。研究了缓冲能力、辅离子的流动性和预处理溶液对该方法稳健性的影响。使用 pH 值为 2.3 的 100 mM 磷酸和 55 mM 三乙醇胺背景电解液，并用 1 M NaOH 进行毛细管预处理，可以分离出所有五种维生素，且分辨率良好。方法的初步验证数据显示，未稀释样品的准确回收率为 94-104%，迁移时间和峰面积的重复性分别在 0.4% RSD 和 2.6% RSD 范围内。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis.

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Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis.

This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10-110 µM for undiluted samples, with 10 μM being the lower range limit of quantification QL, showed accuracy recoveries of 94-104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.