硫酸镁激活l -精氨酸/NO/cGMP通路诱导小鼠外周抗伤感受

IF 1.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Loyara Rocha Miranda Teixeira, Andrea Castro Perez, Thiago Roberto Lima Romero, Igor Dimitri Gama Duarte
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引用次数: 0

摘要

在本研究中,我们研究了硫酸镁是否激活l -精氨酸/NO/cGMP通路并引发外周抗伤感觉。用前列腺素E2足底注射致痛觉过敏的雄性瑞士小鼠进行足爪压力试验。所有药物均局部注入动物右后爪。硫酸镁(20、40、80和160 μg/爪)均有抗伤感受作用。80 μg/爪的剂量可引起局部的抗伤感受作用,该作用可被非选择性NOS抑制剂L-NOArg和选择性神经元NOS抑制剂L-NPA拮抗。抑制剂L-NIO和L-NIL分别选择性抑制内皮细胞和诱导细胞的NOS,但对外周硫酸镁注射无效。可溶性关酰环化酶抑制剂ODQ阻断了硫酸镁的作用,cgmp -磷酸二酯酶抑制剂zaprinast增强了中剂量硫酸镁的抗伤感受作用。我们的研究结果表明,硫酸镁通过神经元NO合成酶刺激NO/cGMP通路,诱导外周抗伤害感受作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Magnesium sulphate activates the L-arginine/NO/cGMP pathway to induce peripheral antinociception in mice

In the present study, we investigated whether magnesium sulphate activates the L-arginine/NO/cGMP pathway and elicits peripheral antinociception. The male Swiss mice paw pressure test was performed with hyperalgesia induced by intraplantar injection of prostaglandin E2. All drugs were administered locally into the right hind paw of animals. Magnesium sulphate (20, 40, 80 and 160 μg/paw) induced an antinociceptive effect. The dose of 80 μg/paw elicited a local antinociceptive effect that was antagonized by the non-selective NOS inhibitor, L-NOArg, and by the selective neuronal NOS inhibitor, L-NPA. The inhibitors, L-NIO and L-NIL, selectively inhibited endothelial and inducible NOS, respectively, but were ineffective regarding peripheral magnesium sulphate injection. The soluble guanylyl cyclase inhibitor, ODQ, blocked the action of magnesium sulphate, and the cGMP-phosphodiesterase inhibitor, zaprinast, enhanced the antinociceptive effects of intermediate dose of magnesium sulphate. Our results suggest that magnesium sulphate stimulates the NO/cGMP pathway via neuronal NO synthase to induce peripheral antinociceptive effects.

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来源期刊
Magnesium research
Magnesium research 医学-内分泌学与代谢
CiteScore
3.50
自引率
9.40%
发文量
6
审稿时长
>12 weeks
期刊介绍: Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.
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