从病猪体内分离出的猪放线杆菌在系统发育上是相关的,但其基因组中的毒素基因拷贝数不同。

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2022-10-03 eCollection Date: 2022-12-01 DOI:10.1002/vro2.45
Dharmasiri Gamage Ruwini Sulochana Kulathunga, Alaa Abou Fakher, Matheus de Oliveira Costa
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引用次数: 0

摘要

目的:革兰氏阴性杆菌猪放线杆菌是一种对养猪业具有全球重要性的病原体,也是导致不同年龄猪只发生致命性呼吸道疾病或败血病的原因。2018 年至 2019 年期间,加拿大西部的 7 个商业农场出现了生长猪感染猪放线杆菌导致死亡率上升的情况。这项工作的目的是用分子方法分析从病猪体内分离出的猪痢疾杆菌,并将其与其他分离物进行比较:这项推论性观察研究使用了从病猪肺部(6 株)、心脏(2 株)和脑部(1 株)获得的 9 株加拿大西部菌株,并进行了全基因组测序。进行了基因组比较分析,以确定存在的遗传变异、抗菌药耐药性和毒力基因的特征:结果:与参考菌株(ATCC 33415)相比,从无粘膜表面的器官中分离出的菌株中发现了更多的 RTX(结构毒素中的重复)基因拷贝,因此理论上更难入侵。加拿大西部的菌株没有携带与猪生产中使用的抗菌剂耐药性有关的基因。在 9 个菌株中的 5 个菌株的基因组中还发现了新的区域,这表明了新菌株的重组和出现:结论:这项研究的结果与新品系的出现有关。RTX毒素基因拷贝数的增加与毒力增强有关。这项研究将有助于加深我们对猪痢疾杆菌的了解,并有助于指导疫苗开发和病原体控制措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<i>Actinobacillus suis</i> isolated from diseased pigs are phylogenetically related but harbour different number of toxin gene copies in their genomes.

<i>Actinobacillus suis</i> isolated from diseased pigs are phylogenetically related but harbour different number of toxin gene copies in their genomes.

<i>Actinobacillus suis</i> isolated from diseased pigs are phylogenetically related but harbour different number of toxin gene copies in their genomes.

Actinobacillus suis isolated from diseased pigs are phylogenetically related but harbour different number of toxin gene copies in their genomes.

Objective: The Gram-negative bacterium Actinobacillus suis is an agent of global importance to the swine industry and the cause of lethal respiratory or septicaemic disease in pigs of different ages. Between 2018 and 2019, seven commercial farms in western Canada experienced episodes of increased mortality due to A. suis infection in grower pigs. The goal of this work was to profile, with molecular methods, A. suis isolated from diseased pigs and to compare them to other isolates.

Design: This inferential observational study used nine western Canadian strains obtained from diseased lungs (n = 6), heart (n = 2) and brain (n = 1) and whole genome sequencing was performed. Comparative genomic analyses were performed to characterise the genetic variability, antimicrobial resistance and the virulence genes present.

Results: Compared to the reference strain (ATCC 33415), an increased number of RTX (repeats in the structural toxin) gene copies were identified in strains isolated from organs without a mucosal surface, thus theoretically harder to invade. Western Canadian strains did not harbour genes associated with resistance to antimicrobial agents used in swine production. Novel regions were also identified in the genomes of five of nine strains demonstrating recombination and emergence of novel strains.

Conclusions: The results obtained in this study were associated with the emergence of new lineages. An increased number of RTX toxin gene copies is suggested to be associated with increased virulence. This study will contribute to improve our understanding regarding A. suis and may help guide vaccine development and agent control measures.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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