Meng-Meng Li , Ai-Ping Lv , Zi-Yu Zhao , Wen-Dong Xian , Zheng-Han Lian , Yu-Ting OuYang , Hong Ming , Sha Tan , Jian-Yu Jiao , En-Min Zhou , Lan Liu , Wen-Jun Li
{"title":"Thermus属五种新的嗜热物种的描述：从温泉沉积物中分离的Thermus hydthermissp.nov.、Thermus neutrimicus sp.nov..、Thermus thalpophilus sp.no..、Thermus-albus sp.nov..和Thermus altitudinis sp.nov.nov。","authors":"Meng-Meng Li , Ai-Ping Lv , Zi-Yu Zhao , Wen-Dong Xian , Zheng-Han Lian , Yu-Ting OuYang , Hong Ming , Sha Tan , Jian-Yu Jiao , En-Min Zhou , Lan Liu , Wen-Jun Li","doi":"10.1016/j.syapm.2022.126361","DOIUrl":null,"url":null,"abstract":"<div><p>Biological denitrification is a significant process in nitrogen biogeochemical cycle of terrestrial geothermal environments, and <em>Thermus</em> species have been shown to be crucial heterotrophic denitrifier in hydrothermal system. Five Gram-stain negative, aerobic and rod-shaped thermophilic bacterial strains were isolated from hot spring sediments in Tibet, China. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences indicated that these isolates should be assigned to the genus <em>Thermus</em> and were most closely related to <em>Thermus caldifontis</em> YIM 73026<sup>T</sup>, and <em>Thermus brockianus</em> YS38<sup>T</sup>. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the five strains and the type strains of the genus <em>Thermus</em> were lower than the threshold values (95% and 70%, respectively) recommended for bacterial species, which clearly distinguished the five isolates from other species of the genus <em>Thermus</em> and indicated that they represent independent species. Colonies are circular, convex, non-transparent. Cell growth occurred at 37–80 °C (optimum, 60–65 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0% (w/v) NaCl (optimum, 0–0.5%). Denitrification genes (<em>narG</em>, <em>nirK</em>, <em>nirS</em>, and <em>norB</em> genes) detected in their genomes indicated their potential function in nitrogen metabolism. The obtained results combined with those of morphological, physiological, and chemotaxonomic characteristics, including the menaquinones, polar lipids, and cellular fatty acids showed that the isolates are proposed as representing five novel species of the genus <em>Thermus</em>, which are proposed as <em>Thermus hydrothermalis</em> sp. nov. SYSU G00291<sup>T</sup>, <em>Thermus neutrinimicus</em> sp. nov. SYSU G00388<sup>T</sup>, <em>Thermus thalpophilus</em> sp. nov. SYSU G00506<sup>T</sup>, <em>Thermus albus</em> sp. nov. SYSU G00608<sup>T</sup>, <em>Thermus altitudinis</em> sp. nov. SYSU G00630<sup>T</sup>.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Description of five novel thermophilic species of the genus Thermus: Thermus hydrothermalis sp. nov., Thermus neutrinimicus sp. nov., Thermus thalpophilus sp. nov., Thermus albus sp. nov., and Thermus altitudinis sp. nov., isolated from hot spring sediments\",\"authors\":\"Meng-Meng Li , Ai-Ping Lv , Zi-Yu Zhao , Wen-Dong Xian , Zheng-Han Lian , Yu-Ting OuYang , Hong Ming , Sha Tan , Jian-Yu Jiao , En-Min Zhou , Lan Liu , Wen-Jun Li\",\"doi\":\"10.1016/j.syapm.2022.126361\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Biological denitrification is a significant process in nitrogen biogeochemical cycle of terrestrial geothermal environments, and <em>Thermus</em> species have been shown to be crucial heterotrophic denitrifier in hydrothermal system. Five Gram-stain negative, aerobic and rod-shaped thermophilic bacterial strains were isolated from hot spring sediments in Tibet, China. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences indicated that these isolates should be assigned to the genus <em>Thermus</em> and were most closely related to <em>Thermus caldifontis</em> YIM 73026<sup>T</sup>, and <em>Thermus brockianus</em> YS38<sup>T</sup>. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the five strains and the type strains of the genus <em>Thermus</em> were lower than the threshold values (95% and 70%, respectively) recommended for bacterial species, which clearly distinguished the five isolates from other species of the genus <em>Thermus</em> and indicated that they represent independent species. Colonies are circular, convex, non-transparent. Cell growth occurred at 37–80 °C (optimum, 60–65 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0% (w/v) NaCl (optimum, 0–0.5%). Denitrification genes (<em>narG</em>, <em>nirK</em>, <em>nirS</em>, and <em>norB</em> genes) detected in their genomes indicated their potential function in nitrogen metabolism. The obtained results combined with those of morphological, physiological, and chemotaxonomic characteristics, including the menaquinones, polar lipids, and cellular fatty acids showed that the isolates are proposed as representing five novel species of the genus <em>Thermus</em>, which are proposed as <em>Thermus hydrothermalis</em> sp. nov. SYSU G00291<sup>T</sup>, <em>Thermus neutrinimicus</em> sp. nov. SYSU G00388<sup>T</sup>, <em>Thermus thalpophilus</em> sp. nov. SYSU G00506<sup>T</sup>, <em>Thermus albus</em> sp. nov. SYSU G00608<sup>T</sup>, <em>Thermus altitudinis</em> sp. nov. SYSU G00630<sup>T</sup>.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202022000686\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202022000686","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Description of five novel thermophilic species of the genus Thermus: Thermus hydrothermalis sp. nov., Thermus neutrinimicus sp. nov., Thermus thalpophilus sp. nov., Thermus albus sp. nov., and Thermus altitudinis sp. nov., isolated from hot spring sediments
Biological denitrification is a significant process in nitrogen biogeochemical cycle of terrestrial geothermal environments, and Thermus species have been shown to be crucial heterotrophic denitrifier in hydrothermal system. Five Gram-stain negative, aerobic and rod-shaped thermophilic bacterial strains were isolated from hot spring sediments in Tibet, China. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences indicated that these isolates should be assigned to the genus Thermus and were most closely related to Thermus caldifontis YIM 73026T, and Thermus brockianus YS38T. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the five strains and the type strains of the genus Thermus were lower than the threshold values (95% and 70%, respectively) recommended for bacterial species, which clearly distinguished the five isolates from other species of the genus Thermus and indicated that they represent independent species. Colonies are circular, convex, non-transparent. Cell growth occurred at 37–80 °C (optimum, 60–65 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0% (w/v) NaCl (optimum, 0–0.5%). Denitrification genes (narG, nirK, nirS, and norB genes) detected in their genomes indicated their potential function in nitrogen metabolism. The obtained results combined with those of morphological, physiological, and chemotaxonomic characteristics, including the menaquinones, polar lipids, and cellular fatty acids showed that the isolates are proposed as representing five novel species of the genus Thermus, which are proposed as Thermus hydrothermalis sp. nov. SYSU G00291T, Thermus neutrinimicus sp. nov. SYSU G00388T, Thermus thalpophilus sp. nov. SYSU G00506T, Thermus albus sp. nov. SYSU G00608T, Thermus altitudinis sp. nov. SYSU G00630T.
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