{"title":"黄花蒿的种系转化。植物通过植物转化技术“花浸”","authors":"Firdaus Qamar, Shazia Khan, Kudsiya Ashrafi, Sadia Iqrar, Syed Naved Quadri, Monica Saifi, M.Z. Abdin","doi":"10.1016/j.btre.2022.e00761","DOIUrl":null,"url":null,"abstract":"<div><p>The therapeutic efficacy of <em>Artemisia annua</em> L. is governed by artemisinin (ART), prevalently produced by <em>A. annua</em> extraction. Due to the modest amount of ART (0.01-1 %dw) in this plant, commercialization of ACTs is difficult. In this study, the floral-dip based transformation protocol for <em>A. annua</em> was developed to enhance expression of artemisinin biosynthesis genes and ART content. For dipping, the effective infiltration media components were optimized, and to obtain high transformation (26.9%) partially open bud stage capitulum of floral development was used. Hygromycin phospho-transferase (<em>hptII</em>) selection marker was used to validate the transformed T<sub>1</sub> progenies. The copy numbers of the transgene (<em>hptII</em>) in T<sub>1</sub> progenies were determined using a sensitive, high-throughput SYBR Green based quantitative RT-PCR. The results of the <em>hptII</em> transgene were compared with those of the low copy number, internal standard (<em>hmgr</em>). Using optimised PCR conditions, one, two and three transgene copies in T<sub>1</sub> transformants were achieved.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/55/main.PMC9489500.pdf","citationCount":"1","resultStr":"{\"title\":\"Germline transformation of Artemisia annuaL. plant via in planta transformation technology “Floral dip”\",\"authors\":\"Firdaus Qamar, Shazia Khan, Kudsiya Ashrafi, Sadia Iqrar, Syed Naved Quadri, Monica Saifi, M.Z. Abdin\",\"doi\":\"10.1016/j.btre.2022.e00761\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The therapeutic efficacy of <em>Artemisia annua</em> L. is governed by artemisinin (ART), prevalently produced by <em>A. annua</em> extraction. Due to the modest amount of ART (0.01-1 %dw) in this plant, commercialization of ACTs is difficult. In this study, the floral-dip based transformation protocol for <em>A. annua</em> was developed to enhance expression of artemisinin biosynthesis genes and ART content. For dipping, the effective infiltration media components were optimized, and to obtain high transformation (26.9%) partially open bud stage capitulum of floral development was used. Hygromycin phospho-transferase (<em>hptII</em>) selection marker was used to validate the transformed T<sub>1</sub> progenies. The copy numbers of the transgene (<em>hptII</em>) in T<sub>1</sub> progenies were determined using a sensitive, high-throughput SYBR Green based quantitative RT-PCR. The results of the <em>hptII</em> transgene were compared with those of the low copy number, internal standard (<em>hmgr</em>). Using optimised PCR conditions, one, two and three transgene copies in T<sub>1</sub> transformants were achieved.</p></div>\",\"PeriodicalId\":38117,\"journal\":{\"name\":\"Biotechnology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/55/main.PMC9489500.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2215017X22000595\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2215017X22000595","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
Germline transformation of Artemisia annuaL. plant via in planta transformation technology “Floral dip”
The therapeutic efficacy of Artemisia annua L. is governed by artemisinin (ART), prevalently produced by A. annua extraction. Due to the modest amount of ART (0.01-1 %dw) in this plant, commercialization of ACTs is difficult. In this study, the floral-dip based transformation protocol for A. annua was developed to enhance expression of artemisinin biosynthesis genes and ART content. For dipping, the effective infiltration media components were optimized, and to obtain high transformation (26.9%) partially open bud stage capitulum of floral development was used. Hygromycin phospho-transferase (hptII) selection marker was used to validate the transformed T1 progenies. The copy numbers of the transgene (hptII) in T1 progenies were determined using a sensitive, high-throughput SYBR Green based quantitative RT-PCR. The results of the hptII transgene were compared with those of the low copy number, internal standard (hmgr). Using optimised PCR conditions, one, two and three transgene copies in T1 transformants were achieved.
Biotechnology ReportsImmunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
15.80
自引率
0.00%
发文量
79
审稿时长
55 days
期刊介绍:
Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.