微管切断蛋白Fidgetin-like 1促进小鼠卵母细胞减数分裂过程中的纺锤体组织。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI:10.1017/S0967199422000417
Hua-Feng Shou, Zhen Jin, Yan Yu, Yu-Cheng Lai, Qing Wu, Lei-Lei Gao
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引用次数: 2

摘要

微管切断蛋白(MTSPs)在有丝分裂和间期中起着重要作用。然而,据我们所知,之前没有研究评估过mtsp在哺乳动物雌性减数分裂中的作用。本研究发现,在小鼠卵母细胞减数分裂过程中,MTSPs成员FIGNL1主要在卵母细胞中表达,并分布在纺锤极。FIGNL1与微管组织中心(MTOC)的重要组成部分γ-微管蛋白共定位并相互作用。特异性小干扰RNA敲低Fignl1导致纺锤体缺陷,其特征是长宽比异常,微管密度降低,从而导致染色体排列异常,卵母细胞成熟和受精障碍。综上所述,本研究结果表明,FIGNL1可能通过纺锤体的形成影响卵母细胞的减数分裂过程,从而成为卵母细胞成熟的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microtubule-severing protein Fidgetin-like 1 promotes spindle organization during meiosis of mouse oocytes.

Microtubule-severing proteins (MTSPs) play important roles in mitosis and interphase. However, to the best of our knowledge, no previous studies have evaluated the role of MTSPs in female meiosis in mammals. It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. FIGNL1 was co-localized and interacted with γ-tubulin, an important component of the microtubule tissue centre (MTOC). Fignl1 knockdown by specific small interfering RNA caused spindle defects characterized by an abnormal length:width ratio and decreased microtubule density, which consequently led to aberrant chromosome arrangement, oocyte maturation and fertilization obstacles. In conclusion, the present results suggested that FIGNL1 may be an essential factor in oocyte maturation by influencing the meiosis process via the formation of spindles.

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来源期刊
Zygote
Zygote 生物-发育生物学
CiteScore
1.70
自引率
5.90%
发文量
117
审稿时长
6-12 weeks
期刊介绍: An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies on gametogenesis through fertilization to gastrulation in animals and humans. The scope has been expanded to include clinical papers, molecular and developmental genetics. The editors will favour work describing fundamental processes in the cellular and molecular mechanisms of animal development, and, in particular, the identification of unifying principles in biology. Nonetheless, new technologies, review articles, debates and letters will become a prominent feature.
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