沙门氏菌提取物通过调节前列腺癌细胞的细胞周期阻滞和细胞凋亡抑制细胞增殖。

IF 1.4 4区 医学 Q4 PHYSIOLOGY
Pang-Ting Cheng, Yu-Chiao Cheng, Muhammet Oner, Yu-Hsuan Li, Mei-Chih Chen, Jyh-Horng Wu, Ting-Chieh Chang, Ayse Celik, Fang-Ling Liu, Hsin-Yi Wang, Chih-Ho Lai, Jer-Tsong Hsieh, Chieh-Yin Chen, Ho Lin
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引用次数: 1

摘要

沙门氏菌(Antrodia salmonea, AS)是一种真菌,属于台湾沙门氏菌真菌科,具有抗氧化、抗炎、抗癌等特点。最近的研究表明,AS对卵巢癌和乳腺癌具有抗癌作用。然而,AS对前列腺癌(PCa)增殖的影响尚不清楚。因此,我们研究了AS在PCa细胞系中通过凋亡和细胞周期调节在PCa增殖中的作用。结果表明,沙门氏菌Antrodia salmonea extract (ASE)对PCa细胞的抑制作用呈剂量依赖性。此外,ASE降低了PC3细胞不依赖于锚定的生长形成能力。此外,ase诱导的PCa细胞(DU145, PC3)的细胞生长抑制与细胞周期相关蛋白如周期蛋白A/B和周期蛋白依赖性激酶CDK1/2/4的减少以及细胞周期抑制剂蛋白p21的增加有关。此外,ASE降低了两种PCa细胞中表皮生长因子受体及其下游信号通路Akt和Erk的总蛋白水平。我们发现,DU145细胞中凋亡标志物如cleaved-PARP蛋白水平显著升高,表明ASE可能诱导细胞凋亡。总之,我们的研究结果表明,ASE可能通过调节细胞周期阻滞和凋亡途径诱导PCa细胞死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antrodia salmonea extract inhibits cell proliferation through regulating cell cycle arrest and apoptosis in prostate cancer cell lines.

Antrodia salmonea (AS) is a fungus, which belongs to a fungal family of Taiwanofungus salmoneus with the features of anti-oxidant, anti-inflammatory, and anticancer. Recent studies have shown that AS has anti-cancer functions in ovarian and breast cancer. However, the effects of AS on prostate cancer (PCa) proliferation remain unknown. Therefore, we investigated the role of AS in PCa proliferation through apoptosis, and cell cycle regulation in PCa cell lines. Our results showed that Antrodia salmonea extract (ASE) inhibited PCa cells growth with a dose-dependent manner. In addition, ASE decreased the anchorage-independent growth formation ability in PC3 cells. Moreover, ASE-induced cell growth inhibition in PCa cells (DU145, PC3) was correlated to decreased cell cycle-related proteins such as cyclin A/B and cyclin-dependent kinase CDK1/2/4, and increased cell cycle inhibitor proteins p21. Besides, ASE decreased the total protein level of epidermal growth factor receptor and its downstream signaling pathways Akt and Erk in both PCa cells. We found that apoptotic markers such as cleaved-PARP protein levels increased significantly in DU145 cells indicating ASE might induce apoptosis. In conclusion, our results suggest that ASE may have the ability to induce PCa cell death through regulating cell cycle arrest and apoptosis pathways.

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来源期刊
CiteScore
2.30
自引率
5.60%
发文量
36
审稿时长
6-12 weeks
期刊介绍: Chinese Journal of Physiology is a multidisciplinary open access journal. Chinese Journal of Physiology (CJP) publishes high quality original research papers in physiology and pathophysiology by authors all over the world. CJP welcomes submitted research papers in all aspects of physiology science in the molecular, cellular, tissue and systemic levels. Multidisciplinary sciences with a focus to understand the role of physiology in health and disease are also encouraged. Chinese Journal of Physiology accepts fourfold article types: Original Article, Review Article (Mini-Review included), Short Communication, and Editorial. There is no cost for readers to access the full-text contents of publications.
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