阳离子树状大分子与阴离子模型生物膜之间的静电相互作用

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Khawla Qamhieh , Tommy Nylander
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引用次数: 1

摘要

采用基于两种不同软球的分析模型,预测了G3、G4和G6代阳离子型聚氨基胺(PAMAM)树状大分子与净阴离子型生物膜之间的静电相互作用。研究了双层表面电荷密度、离子强度、pH、温度、膜柔软度(以双层厚度变化为模型)和树突分子生成对吸引相互作用的影响。随着盐浓度的增加、树突分子电荷的增加和膜的厚度(或柔软度)的增加,这种吸引力减小。另一方面,随着膜的表面电荷密度和树突状分子生成的大小,膜的吸引力增加。事实上,这种吸引力被发现在大世代中要大得多,比如具有更高电荷的G6树状大分子,而在小世代中,比如G3和G4树状大分子。这些结果对利用PAMAM树突状分子作为潜在的基因转染载体具有启示意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrostatic interactions between cationic dendrimers and anionic model biomembrane

The electrostatic interactions between cationic poly(amidoamine) (PAMAM) dendrimers of different generations, G3, G4, and G6, with net anionic model biomembranes have been predicted by adopting an analytical model based on two dissimilar soft spheres. The influence of bilayer surface charge density, ionic strength, pH, temperature, membrane softness (modeled as changes in bilayer thickness), and dendrimer generation on the attractive interaction was investigated. The attraction was found to decrease with increasing salt concentration, dendrimer charge, and thickness (or softness) of the membrane. On the other hand, the attraction increased with the surface charge density of the membrane, and the size of dendrimer generation. In fact, the attraction was found to be much larger for large generations, like G6 dendrimer that have a higher charge, than it is with small ones like G3 and G4 dendrimers. These results have implications for the use of PAMAM dendrimers as potential gene transfection vectors.

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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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