所选双酚类物质对不同胆固醇含量模型红细胞膜的影响

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Karolina Olechowska, Nikola Mielniczek, Katarzyna Hąc-Wydro
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引用次数: 1

摘要

双酚类物质属于已证实对人体红细胞有害的一类环境污染物。然而,这些物质的确切作用可能取决于细胞膜的脂质组成,因为细胞膜结构是细胞内部和外部环境之间的第一道屏障。本研究旨在分析双酚A (BPA)、双酚S (BPS)及其1:1混合物对由鞘磷脂(SM)、胆碱(PC)和胆固醇(Chol)组成的模型人红细胞膜的影响。由于假定生物膜中胆固醇含量与双酚的毒性作用之间存在相关性,研究中使用了不同固醇浓度(10,20和40 mol%的胆固醇)的模型系统。实验采用朗缪尔单层技术和布鲁斯特角度显微镜对脂质体性质进行了研究。研究结果表明,在甾醇含量的研究范围内,BPA的影响,即改变模型膜的组织和稳定性以及削弱脂质相互作用,强烈依赖于系统中Chol的浓度。甾醇含量越高,bpa诱导的膜性质改变越强。然而,考虑到先前报告的含有33.3%胆固醇的系统的结果,似乎BPA的影响与胆固醇含量之间的关系并不是线性的,因为胆固醇浓度较高。相比之下,BPS对模型红细胞膜的影响要弱得多,并且对所研究的系统没有选择性作用。双酚a和双酚a混合物的效果介于单独使用双酚a和双酚a之间,然而,所观察到的效果似乎仅由系统中双酚a的存在决定。因此,取决于年龄或健康状况等因素的人红细胞膜中胆固醇的浓度可能在BPA的毒性作用中起关键作用,而不是BPS。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of selected bisphenols on model erythrocyte membranes of different cholesterol content

Bisphenols belong to the group of environmental pollutants with proven harmful impact on human red blood cells. However, the exact effect of these substances may vary depending on the lipid composition of the cell membrane, since this structure is the first barrier between the cell interior and the external environment. The aim of this work was to analyze the influence of bisphenol A (BPA), bisphenol S (BPS) and their 1:1 mixture on model human erythrocyte membranes, composed of sphingomyelin (SM), phospatidylcholine (PC) and cholesterol (Chol). Due to the postulated correlation between the content of cholesterol in biomembranes and the toxic effect of bisphenols the model systems of different sterol concentrations (10, 20 and 40 mol% of Chol) were used in the studies. In the experiments, Langmuir monolayer technique accompanied with Brewster Angle Microscopy were applied and liposome properties were investigated. The obtained findings reveal that, in the investigated range of the sterol content, the effect of BPA, namely the changes of the organization and stability of model membranes and weakening of the attractive lipid-lipid interactions, is strongly dependent on the concentration of Chol in the system. The higher the sterol content, the stronger the BPA-induced alterations in membrane properties. However taking into account the results reported previously for the system containing 33.3% of cholesterol, it seems that the relationship between the effect of BPA and the amount of Chol is not linear for higher sterol concentrations. In contrast, BPS shows a much weaker influence on model erythrocyte membranes and does not act selectively on the systems studied. The effect of a mixture of BPA and BPS is intermediate between that of BPA and BPS used separately, however, the observed effects appear to be determined only by the presence of BPA in the system. Thus, the concentration of cholesterol in human erythrocyte membranes, which depends on factors such as age or health status, may play a key role in the toxic effects of BPA but not BPS.

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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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