阿瓦斯汀对翼状胬肉成纤维细胞迁移和侵袭的影响。

眼科学报 Pub Date : 2014-12-01

Wei Liu, Xiangyin Sha, Ye Wen, Wenting Zhao, Wei Luo, Zanmei Hua

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引用次数: 0

摘要

目的:探讨阿瓦斯汀对人翼状胬肉成纤维细胞迁移和侵袭性的影响。方法:采用ELISA法测定VEGF- a，比较人翼状胬肉成纤维细胞和结膜成纤维细胞的VEGF分泌情况。通过创面划伤和Transwell迁移试验观察阿瓦斯汀对HPF迁移和侵袭性的影响。western blotting分析p-ERK1/2和p-FAK的表达。结果:(1)人翼状胬肉成纤维细胞比结膜成纤维细胞分泌更多的VEGF。(2)阿瓦斯汀治疗可降低HPF的迁移和侵袭。(3)阿瓦斯汀显著降低人翼状胬肉成纤维细胞中p-ERK1/2和p-FAK的表达。结论:阿瓦斯汀可通过降低p-ERK1/2和p-FAK的表达来抑制HPFs的迁移和侵袭。

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Effect of Avastin on the migration and invasion of pterygium fibroblasts.

Purpose: To evaluate the effect of Avastin on human pterygium fibroblast migration and invasiveness.

Methods: VEGF secretion was compared between human pterygium fibroblasts and conjunctival fibroblasts by measuring VEGF-A by ELISA. The influence of Avastin on HPF migration and invasiveness was observed by wound scratch and Transwell migration assays. The expression of p-ERK1/2 and p-FAK was analyzed by western blotting.

Results: (1)VEGF was secreted in higher amounts by human pterygium fibroblasts than by conjunctival fibroblasts. (2) Avastin treatment decreased HPF migration and invasion. (3) Avastin significantly decreased the expression of p-ERK1/2 and p-FAK in human pterygium fibroblasts.

Conclusion: Avastin can inhibit migration and invasion of HPFs by decreasing the expression of p-ERK1/2 and p-FAK.

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来源期刊

眼科学报

自引率

0.00%

发文量

1312

期刊介绍： Eye science was founded in 1985. It is a national medical journal supervised by the Ministry of Education of the People's Republic of China, sponsored by Sun Yat-sen University, and hosted by Sun Yat-sen University Zhongshan Eye Center (in October 2020, it was changed from a quarterly to a monthly, with the publication number: ISSN: 1000-4432; CN: 44-1119/R). It is edited by Ge Jian, former dean of Sun Yat-sen University Zhongshan Eye Center, Liu Yizhi, director and dean of Sun Yat-sen University Zhongshan Eye Center, and Lin Haotian, deputy director of Sun Yat-sen University Zhongshan Eye Center, as executive editor. It mainly reports on new developments and trends in the field of ophthalmology at home and abroad, focusing on basic research in ophthalmology, clinical experience, and theoretical knowledge and technical operations related to epidemiology. It has been included in important databases at home and abroad, such as Chemical Abstract (CA), China Journal Full-text Database (CNKI), China Core Journals (Selection) Database (Wanfang), and Chinese Science and Technology Journal Database (VIP).