角膜移植术后角膜炎症与角膜淋巴管生成的关系。

眼科学报 Pub Date : 2014-06-01

Weihua Li, Wencong Wang, Shiqi Ling

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引用次数: 0

摘要

目的:探讨角膜移植术后角膜炎症与角膜淋巴管生成的关系。方法:采用淋巴血管内皮受体(LYVE-1)免疫组化和全载免疫荧光法，分别于角膜移植后1、3、7、10、14 d观察大鼠角膜淋巴管生成情况。同时采用炎症指数(IF)分级及NF-κB免疫组化评价角膜炎症。然后检查淋巴管计数(LVC)与IF评分之间的关系。结果:LYVE-1阳性淋巴管在角膜移植后第3天出现在角膜基质中，在第7天和第10天出现，在角膜移植后第14天达到高峰。角膜炎症在移植后第3天强烈，随后逐渐消退，但在移植后第7 ~ 14天再次加重。角膜移植术后LVC与IF呈显著正相关(r = 0.41;P < 0.05)。然而，IF评分和LVC的变化并不平行。结论:角膜移植术后角膜淋巴管生成与角膜炎症有密切而非平行的关系。

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An association between corneal inflammation and corneal lymphangiogenesis after keratoplasty.

Purpose: To examine the relationship between corneal inflammation and corneal lymphangiogenesis after keratoplasty.

Methods: Rat corneal lymphangiogenesis was examined by lymphatic vessel endothelial receptor (LYVE-1) immunohistochemistry and whole mount immunofluorescence at 1, 3, 7, 10, and 14 days after corneal transplantation. Corneal inflammation was evaluated by inflammation index (IF) grading and NF-κB immunohistochemistry at the same time points. The association between lymphatic vessel counting (LVC) and the IF scores was then examined.

Results: LYVE-1 positive lymphatic vessels occurred in the corneal stroma on day 3, developed throughout days 7 and 10, and peaked in number at day 14 after keratoplasty. Corneal inflammation was strong on day 3, and then resolved gradually, but increased again from days 7 to 14 after the transplantation. LVC was strongly and positively correlated with IF after keratoplasty (r = 0.41; P < 0.05). However, changes in IF scores and LVC were not parallel.

Conclusion: A close, but not parallel, relationship was found between corneal lymphangiogenesis and corneal inflammation after corneal transplantation.

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来源期刊

眼科学报

自引率

0.00%

发文量

1312

期刊介绍： Eye science was founded in 1985. It is a national medical journal supervised by the Ministry of Education of the People's Republic of China, sponsored by Sun Yat-sen University, and hosted by Sun Yat-sen University Zhongshan Eye Center (in October 2020, it was changed from a quarterly to a monthly, with the publication number: ISSN: 1000-4432; CN: 44-1119/R). It is edited by Ge Jian, former dean of Sun Yat-sen University Zhongshan Eye Center, Liu Yizhi, director and dean of Sun Yat-sen University Zhongshan Eye Center, and Lin Haotian, deputy director of Sun Yat-sen University Zhongshan Eye Center, as executive editor. It mainly reports on new developments and trends in the field of ophthalmology at home and abroad, focusing on basic research in ophthalmology, clinical experience, and theoretical knowledge and technical operations related to epidemiology. It has been included in important databases at home and abroad, such as Chemical Abstract (CA), China Journal Full-text Database (CNKI), China Core Journals (Selection) Database (Wanfang), and Chinese Science and Technology Journal Database (VIP).