Haiguan Yang, Junqi Zha, Peng Zhang, Yuemei Qin, Tao Chen, Fanggui Ye
{"title":"CeVO4纳米酶的制备及其对苯二酚和儿茶酚的比色鉴别","authors":"Haiguan Yang, Junqi Zha, Peng Zhang, Yuemei Qin, Tao Chen, Fanggui Ye","doi":"10.1016/j.snb.2017.03.042","DOIUrl":null,"url":null,"abstract":"<div><p>A simple method was proposed for the preparation of CeVO<sub>4</sub>. The as-synthesized CeVO<sub>4</sub> was, for the first time, demonstrated to exhibit both peroxidase-like and oxidase-like activity, which catalyzes the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to form a typical blue solution in the presence or absence of H<sub>2</sub>O<sub>2</sub>. Moreover, the mechanism of its dual-enzyme activity was investigated in detail. The Michaelis constant (<em>K</em><sub>m</sub>) value for CeVO<sub>4</sub> (0.136<!--> <!-->mM) was lower than that of horseradish peroxidase (0.424<!--> <!-->mM) with TMB as the substrate. Interestingly, hydroquinone (H<sub>2</sub>Q), dihydroxybenzene isomer, undergoes reduction accompanying the oxidation of TMB by the CeVO<sub>4</sub> oxidase mimic along with a visible color change, while the other two dihydroxybenzene isomers, i.e., resorcinol (RC) and catechol (CC), do not. Based on these findings, a colorimetric platform was developed to discriminate H<sub>2</sub>Q from RC and CC. Under optimal conditions, a linear relationship between the H<sub>2</sub>Q concentration and absorbance was observed from 0.05 to 8<!--> <!-->μM, and a limit of detection of 0.04<!--> <!-->μM was achieved. Moreover, this colorimetric platform can selectively reveal H<sub>2</sub>Q concentrations in the presence of other dihydroxybenzene isomers.</p></div>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.snb.2017.03.042","citationCount":"57","resultStr":"{\"title\":\"Fabrication of CeVO4 as nanozyme for facile colorimetric discrimination of hydroquinone from resorcinol and catechol\",\"authors\":\"Haiguan Yang, Junqi Zha, Peng Zhang, Yuemei Qin, Tao Chen, Fanggui Ye\",\"doi\":\"10.1016/j.snb.2017.03.042\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A simple method was proposed for the preparation of CeVO<sub>4</sub>. The as-synthesized CeVO<sub>4</sub> was, for the first time, demonstrated to exhibit both peroxidase-like and oxidase-like activity, which catalyzes the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to form a typical blue solution in the presence or absence of H<sub>2</sub>O<sub>2</sub>. Moreover, the mechanism of its dual-enzyme activity was investigated in detail. The Michaelis constant (<em>K</em><sub>m</sub>) value for CeVO<sub>4</sub> (0.136<!--> <!-->mM) was lower than that of horseradish peroxidase (0.424<!--> <!-->mM) with TMB as the substrate. Interestingly, hydroquinone (H<sub>2</sub>Q), dihydroxybenzene isomer, undergoes reduction accompanying the oxidation of TMB by the CeVO<sub>4</sub> oxidase mimic along with a visible color change, while the other two dihydroxybenzene isomers, i.e., resorcinol (RC) and catechol (CC), do not. Based on these findings, a colorimetric platform was developed to discriminate H<sub>2</sub>Q from RC and CC. Under optimal conditions, a linear relationship between the H<sub>2</sub>Q concentration and absorbance was observed from 0.05 to 8<!--> <!-->μM, and a limit of detection of 0.04<!--> <!-->μM was achieved. Moreover, this colorimetric platform can selectively reveal H<sub>2</sub>Q concentrations in the presence of other dihydroxybenzene isomers.</p></div>\",\"PeriodicalId\":8,\"journal\":{\"name\":\"ACS Biomaterials Science & Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2017-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.snb.2017.03.042\",\"citationCount\":\"57\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Biomaterials Science & Engineering\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0925400517304598\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925400517304598","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Fabrication of CeVO4 as nanozyme for facile colorimetric discrimination of hydroquinone from resorcinol and catechol
A simple method was proposed for the preparation of CeVO4. The as-synthesized CeVO4 was, for the first time, demonstrated to exhibit both peroxidase-like and oxidase-like activity, which catalyzes the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to form a typical blue solution in the presence or absence of H2O2. Moreover, the mechanism of its dual-enzyme activity was investigated in detail. The Michaelis constant (Km) value for CeVO4 (0.136 mM) was lower than that of horseradish peroxidase (0.424 mM) with TMB as the substrate. Interestingly, hydroquinone (H2Q), dihydroxybenzene isomer, undergoes reduction accompanying the oxidation of TMB by the CeVO4 oxidase mimic along with a visible color change, while the other two dihydroxybenzene isomers, i.e., resorcinol (RC) and catechol (CC), do not. Based on these findings, a colorimetric platform was developed to discriminate H2Q from RC and CC. Under optimal conditions, a linear relationship between the H2Q concentration and absorbance was observed from 0.05 to 8 μM, and a limit of detection of 0.04 μM was achieved. Moreover, this colorimetric platform can selectively reveal H2Q concentrations in the presence of other dihydroxybenzene isomers.
期刊介绍：
ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics:
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