小儿短肠综合征患者的粪便微生物组。

Zev H Davidovics, Beth A Carter, Ruth Ann Luna, Emily B Hollister, Robert J Shulman, James Versalovic
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引用次数: 49

摘要

背景:短肠综合征(SBS)患者肠道微生物组的变化被认为对临床结果有显著影响。这些变化不仅可能延缓肠内饮食的进展,而且可能使患者易发生细菌易位、菌血症和肝脏疾病。SBS患者被认为更容易受到肠道微生物群落变化的影响,这是由于肠道运动障碍和/或缺乏解剖学保护措施,如回盲瓣。材料与方法:采用16S核糖体RNA基因测序法对9例SBS患儿和8例4个月~ 8岁健康儿童的21份粪便标本进行细菌组成分析。使用QIIME、核糖体数据库项目分类器和随机森林监督学习算法对序列进行质量过滤和分析。结果:SBS患者的粪便微生物群与健康对照组存在差异。SBS患者的粪便中细菌种类γ变形杆菌和芽胞杆菌的丰度明显更高。SBS患者排便次数增加,其粪便中乳酸杆菌丰度增加(P = 0.057),瘤胃球菌丰度减少。结论:本研究显示SBS患者的粪便微生物组通过16S宏基因组学分析与健康对照组存在显著差异。SBS患儿肠道微生物组组成和功能的差异可能影响肠道生理,这些发现可能为肠道康复和临床管理提供新的机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The Fecal Microbiome in Pediatric Patients With Short Bowel Syndrome.

The Fecal Microbiome in Pediatric Patients With Short Bowel Syndrome.

The Fecal Microbiome in Pediatric Patients With Short Bowel Syndrome.

The Fecal Microbiome in Pediatric Patients With Short Bowel Syndrome.

Background: Changes in the intestinal microbiome of patients with short bowel syndrome (SBS) are thought to significantly affect clinical outcome. These changes may not only delay enteral diet advancement but may also predispose patients to bacterial translocation, bacteremia, and liver disease. Patients with SBS are thought to be more susceptible to changes in gut microbial communities due to intestinal dysmotility and/or lack of anatomic safeguards such as the ileocecal valve.

Materials and methods: We analyzed the bacterial composition of 21 fecal specimens from 9 children with SBS and 8 healthy children ages 4 months to 8 years by 16S ribosomal RNA gene sequencing. The sequences were quality filtered and analyzed using QIIME, the Ribosomal Database Project Classifier, and the randomForest supervised learning algorithm.

Results: The fecal microbiome of patients with SBS is different from that of healthy controls. Stool from patients with SBS had a significantly greater abundance of the bacterial classes Gammaproteobacteria and Bacilli. Stool from patients with SBS who experienced increased stool frequency tended to have increased abundance of Lactobacillus (P = .057) and decreased abundance of Ruminococcus.

Conclusion: This study shows that the fecal microbiome of patients with SBS is significantly different from that of healthy controls when analyzed by 16S metagenomics. Differences in the composition and function of gut microbiomes in children with SBS may affect bowel physiology, and these findings may provide new opportunities for intestinal rehabilitation and clinical management.

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