血管紧张素转换酶插入/缺失(I/D) (rs4646994)与VEGF多态性(+405G/C;与伊朗2型糖尿病患者蛋白尿的发展协同相关。

IF 0.4 4区 医学
Iranian Red Crescent Medical Journal Pub Date : 2015-02-21 eCollection Date: 2015-02-01 DOI:10.5812/ircmj.19469
Mohammad Fathi, Abdol Rahim Nikzamir, Alireza Esteghamati, Manouchehr Nakhjavani, Mir Saeed Yekaninejad
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引用次数: 4

摘要

背景:血管紧张素转换酶(ACE)插入/缺失(I/D)和血管内皮生长因子(VEGF)多态性与糖尿病肾病(DN)有关。目的:我们研究了ACE-D和VEGF-G等位基因在2型糖尿病(T2DM)患者中与DN相关的协同作用的假设。患者与方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测490例T2DM患者的VEGF (rs2010963)和ACE (rs4646994)基因型。糖尿病患者分为伴有蛋白尿和不伴有蛋白尿的2型糖尿病患者(对照组)。采用PCR和RFLP检测VEGF和ACE等位基因。结果:共纳入255例连续T2DM合并微量白蛋白尿患者(A组)和235例T2DM合并正常白蛋白尿患者(B组)。在单因素分析中,各组除糖化血红蛋白(HbA1c) (P = 0.034)、ACE (P = 0.015)和VEGF (P = 0.006)基因型频率外,其他变量均有统计学差异。我们的研究表明VEGF-G和ACE-D等位基因与肾病的发展独立相关。根据我们的数据,这两个危险因素的组合对微量白蛋白尿发展的风险具有显著的协同作用。结论:我们的研究表明,ACE-D和VEGF-G等位基因可能是T2DM患者微量白蛋白尿的独立危险因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Combination of Angiotensin Converting Enzyme Insertion/Deletion (I/D) (rs4646994) and VEGF Polymorphism (+405G/C; rs2010963) Synergistically Associated With the Development, of Albuminuria in Iranian Patients With Type 2 Diabetes.

Background: Angiotensin-converting enzyme (ACE) insertion/deletion (I/D) and vascular endothelial growth factor (VEGF) polymorphisms have been shown to associate with diabetic nephropathy (DN).

Objectives: We examined the hypothesis that ACE-D and VEGF-G alleles act synergistically in association with DN, in patients with type 2 diabetes mellitus (T2DM).

Patients and methods: The VEGF (rs2010963) and ACE (rs4646994) genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 490 T2DM patients. Diabetic patients were classified as T2DM patients with and without albuminuria (control). The PCR and RFLP were used to detect the VEGF and ACE alleles.

Results: A total of 255 consecutive patients with T2DM and microalbuminuria (Group A) and 235 patients with T2DM and normoalbuminuria (Group B) were included in the study. In univariate analysis, the groups were statistically similar for all variables, except for glycated hemoglobin (HbA1c) (P = 0.034), and the frequency of ACE (P = 0.015) and VEGF (P = 0.006) genotypes. Our study showed that the VEGF-G and ACE-D alleles are independently associated with the development of nephropathy. According to our data, the combination of these two risk factors had a significant synergistic effect on the risk of microalbuminuria development.

Conclusions: Our study indicated that ACE-D and VEGF-G alleles can be an independent risk factor for microalbominuria in T2DM patients.

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来源期刊
Iranian Red Crescent Medical Journal
Iranian Red Crescent Medical Journal 医学-医学:内科
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期刊介绍: The IRANIAN RED CRESCENT MEDICAL JOURNAL is an international, English language, peer-reviewed journal dealing with general Medicine and Surgery, Disaster Medicine and Health Policy. It is an official Journal of the Iranian Hospital Dubai and is published monthly. The Iranian Red Crescent Medical Journal aims at publishing the high quality materials, both clinical and scientific, on all aspects of Medicine and Surgery
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