链脲佐菌素糖尿病大鼠指长伸肌抗氧化酶和脂质过氧化的变化可能导致肌肉萎缩。

Koji Nonaka, S Une, N Tatsuta, K Ito, J Akiyama
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引用次数: 9

摘要

我们研究了链脲霉素糖尿病大鼠指长伸肌(EDL,主要是快纤维)和比目鱼肌(主要是慢纤维)的肌肉萎缩、主要抗氧化酶和脂质过氧化。将雌性Wistar大鼠分为对照组(n = 5)和链脲霉素诱导的糖尿病组(n = 5)。糖尿病诱导8周后,去除EDL和比罗鱼肌,测定过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)活性和硫代巴比妥酸活性物质(TBARS)水平。糖尿病大鼠EDL肌和比目鱼肌的CAT活性均升高(p < 0.01), GPX和SOD活性仅在EDL肌升高(p < 0.01和p < 0.05)。TBARS水平仅在糖尿病大鼠EDL肌中升高(p < 0.01)。两组肌肉均出现明显萎缩,但EDL肌萎缩最严重。综上所述,糖尿病大鼠比目鱼肌对氧化应激的适应性反应是充分的,而EDL肌则不是。因此,快速收缩肌纤维可能比缓慢收缩肌纤维更容易受到氧化应激的影响,这可能导致糖尿病患者的肌肉萎缩。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in antioxidant enzymes and lipid peroxidation in extensor digitorum longus muscles of streptozotocin-diabetic rats may contribute to muscle atrophy.

We investigated muscle atrophy, major antioxidant enzymes and lipid peroxidation in the extensor digitorum longus (EDL, predominantly fast fibers) and soleus (predominantly slow fibers) muscle of streptozotocin-diabetic rats. Female Wistar rats were divided into a control (n = 5) and streptozotocin-induced diabetic group (n = 5). Eight weeks after diabetes induction the EDL and soleus muscles were removed and catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase activity (SOD), and thiobarbituric acid reactive substances (TBARS) levels measured. The CAT activity increased in both the EDL and soleus muscles of the diabetic rats (p < 0.01), whereas the GPX and SOD activities were increased only in the EDL muscle (p < 0.01 and p < 0.05). The TBARS levels were only increased in the EDL muscle of the diabetic rats (p < 0.01). Both muscles showed significant atrophy but the EDL muscle elicited the greatest atrophy. In conclusion, it appears that adaptive responses to oxidative stress were adequate in the soleus muscle, but not in the EDL muscle, of diabetic rats. Thus fast twitch muscle fibers may be more susceptible to oxidative stress than slow twitch muscle fibers and this may contribute to muscle atrophy under diabetic conditions.

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来源期刊
Acta physiologica Hungarica
Acta physiologica Hungarica 医学-生理学
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