胃癌肿瘤和非肿瘤粘膜RET原癌基因产物的免疫组化检测。

IF 0.1 4区 医学 Q4 Medicine
Fan Zhang, Jian Min Tang, Li Wang, Ping Ping Wu, Mei Zhang
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引用次数: 0

摘要

目的:应用免疫组化(IHC)方法检测胃癌组织中RET (rearrange during Transfection)蛋白的表达。研究设计:共210个样本,其中197个样本来自91个胃腺癌手术切片,包括91个肿瘤样本、91个非肿瘤样本和15个粘膜内发育不良样本。另外13个胃粘膜来自无癌患者。两个RET抗体(克隆Ret01和3F8)分别用于免疫组化。结果:在胃癌非肿瘤样本中,28例(31%)抗体Ret01或3F8阳性。阳性染色通常位于幽门深腺,并与慢性炎症模式相关(p = 0.045)。RET阳性与先前检测的表皮生长因子受体磷酸化相关(p = 0.021)。Ret01抗体阳性7例(8%),3F8抗体阳性9例(10%)。在15例粘膜发育不良样本中,Ret01抗体检测到RET 6例,3F8抗体检测到RET 8例。在肿瘤、非肿瘤和粘膜内发育不良样本中,使用抗体Ret01和3F8的免疫反应是一致的(p = 0.500、1.000和0.500)。13例无癌患者的样本均为阴性。结论:RET原癌基因的激活可能是胃炎性和肿瘤疾病的分子发病机制之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical detection of RET proto-oncogene product in tumoral and nontumoral mucosae of gastric cancer.

Objective: To detect RET (REarranged during Transfection) protein by immunohistochemistry (IHC) in gastric cancer.

Study design: A total of 210 samples were employed, of which 197 specimens were from 91 surgical pieces of gastric adenocarcinoma, comprising 91 tumoral, 91 nontumoral, and 15 intramucosal dysplastic samples. Another 13 gastric mucosae were from cancer-free patients. Two RET antibodies (clones Ret01 and 3F8) were used separately for IHC.

Results: Of the nontumoral samples from gastric cancers, 28 were positive (31%) with either antibody Ret01 or 3F8. The positive stains were often located in deep pyloric glands and associated with chronic inflammation patterns (p = 0.045). RET positivity correlated with phosphorylated epidermal growth factor receptor, which had been previously tested (p = 0.021). In tumoral samples RET was positive in 7 cases with antibody Ret01 (8%) and 9 cases with 3F8 (10%). In 15 intramucosal dysplastic samples RET was detected in 6 cases with antibody Ret01 and 8 cases with 3F8. There was an accordance between the IHC using antibodies Ret01 and 3F8 in tumoral, nontumoral, and intramucosal dysplastic samples (p = 0.500, 1.000, and 0.500). The 13 samples from cancer-free patients were always negative.

Conclusion: Activation of RET proto-oncogene may be one of the molecular pathogeneses in gastric inflammatory and tumoral diseases.

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期刊介绍: AQCH is an Official Periodical of The International Academy of Cytology and the Italian Society of Urologic Pathology.
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