单宁酸标记表明异常细胞发育与肾小管再生一致。

Q2 Medicine
BMC Clinical Pathology Pub Date : 2014-07-15 eCollection Date: 2014-01-01 DOI:10.1186/1472-6890-14-34
Will W Minuth, Lucia Denk
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引用次数: 5

摘要

背景:干细胞/祖细胞作为刺激病变肾实质再生的未来治疗选择是研究的焦点。然而,目前的数据表明,植入的干细胞/祖细胞的成功播种受到有害的间质液和改变的细胞外基质的阻碍。为了找出细胞适应的可能参数,进行了本研究。方法:将肾干/祖细胞置于人工间质中进行灌注培养。在13天内测试暴露于化学定义但不依赖二氧化碳的培养基。然后通过组织化学分析细胞生物学特征,同时在常规和改进的标本固定后通过透射电镜研究结构细节。结果:在莱博维茨L-15培养基和CO2不依赖培养基中培养的肾干/祖细胞在荧光显微镜下显示出许多小管的空间发育。用常规戊二醛固定的两种培养基的标本在电子显微镜下显示出发达的小管中均匀的细胞群。相比之下,戊二醛(包括单宁酸)固定表明存在分散的、功能未知的深色标记细胞。筛选进一步表明,暗细胞类型与胚胎、成熟或成熟肾实质中的细胞不一致。结论:实际数据表明,在体外条件下模拟肾小管再生时,必须考虑异常细胞特征的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules.

Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules.

Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules.

Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules.

Background: Stem/progenitor cells are in the focus of research as a future therapeutic option to stimulate regeneration in diseased renal parenchyma. However, current data indicate that successful seeding of implanted stem/progenitor cells is prevented by harmful interstitial fluid and altered extracellular matrix. To find out possible parameters for cell adaptation, the present investigation was performed.

Methods: Renal stem/progenitor cells were mounted in an artificial interstitium for perfusion culture. Exposure to chemically defined but CO2-independent culture media was tested during 13 days. Cell biological features were then analyzed by histochemistry, while structural details were investigated by transmission electron microscopy after conventional and improved fixation of specimens.

Results: Culture of renal stem/progenitor cells as well in Leibovitz's L-15 Medium as CO2 Independent Medium shows in fluorescence microscopy spatial development of numerous tubules. Specimens of both media fixed by conventional glutaraldehyde exhibit in electron microscopy a homogeneous cell population in developed tubules. In contrast, fixation by glutaraldehyde including tannic acid illuminates that dispersed dark marked cells of unknown function are present. The screening further demonstrates that the dark cell type does not comply with cells found in embryonic, maturing or matured renal parenchyma.

Conclusions: The actual data show that development of abnormal cell features must be taken into account, when regeneration of renal tubules is simulated under in vitro conditions.

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来源期刊
BMC Clinical Pathology
BMC Clinical Pathology Medicine-Pathology and Forensic Medicine
CiteScore
3.30
自引率
0.00%
发文量
0
期刊介绍: BMC Clinical Pathology is an open access journal publishing original peer-reviewed research articles in all aspects of histopathology, haematology, clinical biochemistry, and medical microbiology (including virology, parasitology, and infection control). BMC Clinical Pathology (ISSN 1472-6890) is indexed/tracked/covered by PubMed, CAS, EMBASE, Scopus and Google Scholar.
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