低温球蛋白作为精神分裂症炎症的潜在诱因。

IF 3.6 Q1 PSYCHIATRY
Schizophrenia Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-12-15 DOI:10.1155/2013/125264
Andranik Chavushyan, Meri Hovsepyan, Anna Boyajyan
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引用次数: 3

摘要

本研究旨在探讨从精神分裂症患者血液中分离的III型冷球蛋白对外周血单核细胞(PBMCs)产生促炎细胞因子白介素(IL)-1 β、IL-6和肿瘤坏死因子- α (TNF- α)、抗炎细胞因子IL-10、趋化细胞因子IL-8和单核细胞趋化蛋白-1 (MCP-1)的影响。实验采用pbmc健康受试者和精神分裂症患者的血液进行体外实验。采用酶联免疫吸附法和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑测定法。结果表明,从0.4 mg/mL开始,低温球蛋白孵育24小时后,培养的pbmc细胞IL-1 β、IL-6、TNF- α、IL-8和MCP-1的产量显著增加(P < 0.05)。性别差异不影响冷球蛋白诱导pbmc产生这些细胞因子。未观察到低温球蛋白对PBMCs产生IL-10的影响。低温球蛋白浓度≤4 mg/mL对培养的PBMCs无细胞毒作用。基于所获得的结果,我们得出结论,III型冷球蛋白通过诱导促炎和趋化细胞因子的表达,与精神分裂症相关的免疫反应改变有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Cryoglobulins as potential triggers of inflammation in schizophrenia.

Cryoglobulins as potential triggers of inflammation in schizophrenia.

Cryoglobulins as potential triggers of inflammation in schizophrenia.

Cryoglobulins as potential triggers of inflammation in schizophrenia.

This case study aimed to investigate effects of type III cryoglobulins isolated from the blood of patients with schizophrenia on the production of proinflammatory cytokines interleukin(IL)-1 β , IL-6 and tumor necrosis factor- α (TNF- α ), anti-inflammatory cytokine IL-10, and chemotactic cytokines IL-8 and monocyte chemoattractant protein-1 (MCP-1) by peripheral blood mononuclear cells (PBMCs). The experiments were performed in vitro using PBMCs healthy subjects and the blood of patients whit schizoprenia. The enzyme-linked immunosorbent assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay were used upon study. The results obtained indicated significant increase (P < 0.05) in IL-1 β , IL-6, TNF- α , IL-8, and MCP-1 production by cultured PBMCs when incubating for 24 hours with cryoglobulins, beginning from 0.4 mg/mL. The gender difference does not affect the cryoglobulins-induced production of these cytokines by PBMCs. No influence of cryoglobulins on production of IL-10 by PBMCs was observed. Also, it was shown that cryoglobulins in concentration ≤4 mg/mL possessed no cytotoxic effect towards cultured PBMCs. Based upon the results obtained, we concluded that type III cryoglobulins are implicated in schizophrenia-associated alterations in the immune response through induction of the expression of proinflammatory and chemotactic cytokines by PBMCs.

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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
2
审稿时长
14 weeks
期刊介绍: Schizophrenia Research and Treatment is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to all aspects of schizophrenia.
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