聚乙烯醇水凝胶增强细胞粘附的等离子体功能化研究。

Biomatter Pub Date : 2013-10-01 Epub Date: 2013-07-29 DOI:10.4161/biom.25414
Julia M Ino, Pascale Chevallier, Didier Letourneur, Diego Mantovani, Catherine Le Visage
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引用次数: 49

摘要

裁剪生物材料和周围组织之间的界面相互作用是医疗设备设计要考虑的一个重要方面。聚乙烯醇(PVA)水凝胶具有适合于各种生物替代品的力学性能,但其表面缺乏细胞粘附性往往是一个问题。常见的方法是通过混合或偶联的方式将生物分子结合在一起。但这些修饰破坏了PVA分子内和分子间的相互作用,从而导致其原有的机械性能的丧失。在这项工作中,辉光放电等离子体的表面改性技术,已知只修饰表面而不改变体积性质,已经研究了促进细胞附着在聚乙烯醇基板上。进行了N2/H2微波等离子体处理,研究了聚乙烯醇表面的化学成分。等离子体处理膜的x射线光电子和傅里叶变换红外分析显示,PVA的主要结构没有变化,但羰基和氮基团(包括胺和酰胺基团)的存在。等离子体修饰导致PVA表面润湿性增加,而表面粗糙度没有显著变化。与未处理的PVA相比,血浆修饰膜可以成功培养小鼠成纤维细胞和人内皮细胞。结果表明,该接枝在复水化后是稳定的,具有良好的细胞粘附性能。因此,PVA的血浆胺化是一种很有前途的方法,可以改善细胞与合成水凝胶接触时的行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Plasma functionalization of poly(vinyl alcohol) hydrogel for cell adhesion enhancement.

Plasma functionalization of poly(vinyl alcohol) hydrogel for cell adhesion enhancement.

Plasma functionalization of poly(vinyl alcohol) hydrogel for cell adhesion enhancement.

Plasma functionalization of poly(vinyl alcohol) hydrogel for cell adhesion enhancement.

Tailoring the interface interactions between a biomaterial and the surrounding tissue is a capital aspect to consider for the design of medical devices. Poly(vinyl alcohol) (PVA) hydrogels present suitable mechanical properties for various biological substitutes, however the lack of cell adhesion on their surface is often a problem. The common approach is to incorporate biomolecules, either by blending or coupling. But these modifications disrupt PVA intra- and intermolecular interactions leading therefore to a loss of its original mechanical properties. In this work, surface modification by glow discharge plasma, technique known to modify only the surface without altering the bulk properties, has been investigated to promote cell attachment on PVA substrates. N2/H2 microwave plasma treatment has been performed, and the chemical composition of PVA surface has been investigated. X-ray photoelectron and Fourier transform infrared analyses on the plasma-treated films revealed the presence of carbonyl and nitrogen species, including amine and amide groups, while the main structure of PVA was unchanged. Plasma modification induced an increase in the PVA surface wettability with no significant change in surface roughness. In contrast to untreated PVA, plasma-modified films allowed successful culture of mouse fibroblasts and human endothelial cells. These results evidenced that the grafting was stable after rehydration and that it displayed cell adhesive properties. Thus plasma amination of PVA is a promising approach to improve cell behavior on contact with synthetic hydrogels for tissue engineering.

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