伊朗东部亚塞拜然两种马先蒿的抗氧化剂、总酚和类黄酮含量。

BioImpacts : BI Pub Date : 2012-01-01 Epub Date: 2012-03-24 DOI:10.5681/bi.2012.006
Laleh Khodaie, Sedigheh Bamdad, Abbas Delazar, Hossein Nazemiyeh
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引用次数: 37

摘要

简介:马先蒿(Pedicularis sibthorpii)和马先蒿(P. wilhelmsiana)是伊朗西北部的特有种。马先蒿属植物产生一些重要的多酚和类黄酮。本文对上述植物的总酚和类黄酮含量及其抗氧化能力进行了测定。方法:采用固相萃取法(SPE)对样品进行分离,并记录样品的H-NMR谱。采用Folin- Ciocalteu法和氯化铝法测定甲醇提取物的总酚和总黄酮含量。用分光光度法测定了紫外光色1,1 -二苯基吡啶酰肼(DPPH)甲醇溶液的漂白效果,以测定其提取物和馏分的抗氧化活性。结果:马先蒿总酚含量在8 ~ 30 mg g(-1)和9 ~ 20 mg g(-1)之间。西氏木40%、60%和黄颡鱼20%、40%、60%部位酚类化合物含量较高。黄酮含量在0 ~ 215 mg g(-1)和0 ~ 177 mg g(-1)之间,其中40%和60%部位黄酮含量较高。抗氧化活性在0.01 ~ 0.7 mg mL(-1)和0.01 ~ 1.02 mg mL(-1)之间。sibthorpii的20%和40%组分以及wilhelmsiana的40%和60%组分的RC50均低于其他组分。结论:RC50越低,黄酮类化合物和酚类化合物含量越高。核磁共振谱分析结果与上述发现基本一致,表明对西氏和威廉氏进行植物化学研究是有价值的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant, total phenol and flavonoid contents of two pedicularis L. Species from eastern azerbaijan, iran.

Introduction: Pedicularis sibthorpii and P. wilhelmsiana are endemic species mainly found in North-West of Iran. Plants of genus Pedicularis produce some important poly-phenols and flavonoids. In the present work, total phenol and flavonoid contents of the mentioned species as well as their antioxidant capacity have been evaluated.

Methods: Methanol extract of samples was fractionated by SPE method using an ODS cartridge and their (1)H-NMR spectra were recorded. Total phenols and flavonoids of methanol extracts were determined using Folin- Ciocalteu and aluminum chloride methods. For determining antioxidant activity of the extracts and fractions, bleaching of purple color methanol solu-tion of 1, 1-diphenylpycryl hydrazyl (DPPH) was measured by spectrophotometric assay.

Results: Total phenols of Pedicularis sibthorpii and P. wilhelmsiana were in the range of 8-30 mg g(-1) and 9-20 mg g(-1), respectively. The 40% and 60% fractions of P. sibthorpii and the 20%, 40% and 60% fractions of P. wilhelmsiana showed higher amounts of phenolic compounds. The total flavonoid contents of P. sibthorpii and P. wilhelmsiana were in the range of 0-215 mg g(-1) and 0-177 mg g(-1), respectively, whereas the 40% and 60% fractions showed higher flavonoid amounts. Antioxidant activity of P. sibthorpii and P. wil-helmsiana were in the range of 0.01-0.7 mg mL(-1) and 0.01-1.02 mg mL(-1). In the same manner, the 20% and 40% fractions of P. sibthorpii and the 40% and 60% fractions of P. wilhelmsiana had lower RC50 than that of other fractions.

Conclusion: Fractions with lower RC50 had higher contents of phenolic and flavonoid compounds. The results of NMR spectra were parallel with these findings and show that it is worth to do phytochemi-cal studies on P. sibthorpii and P. wilhelmsiana.

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