聚乳酸-羟基乙酸核+聚n -异丙基丙烯酰胺壳纳米粒子体系的合成与表征。

Biomatter Pub Date : 2012-10-01 DOI:10.4161/biom.22494
Aaron M Kosinski, Jamie L Brugnano, Brandon L Seal, Frances C Knight, Alyssa Panitch
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引用次数: 13

摘要

聚乳酸-羟基乙酸(PLGA)是制备纳米药物递送材料的常用材料。然而,PLGA纳米颗粒缺乏理想的特性,包括主动靶向能力,在冻干过程中抵抗聚集,以及对动态环境刺激的响应能力。为了克服这些问题,我们制造了一种由聚n -异丙基丙烯酰胺外壳内包裹的PLGA核心组成的纳米颗粒。采用动态光散射和透射电镜成像对纳米颗粒进行了表征,MTT试验和ELISA检测表明纳米颗粒在THP1细胞中的生物相容性。最后,胶原II型结合试验显示,这些纳米颗粒被成功地修饰为具有活性靶向部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.

Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.

Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.

Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.

Poly(lactic-co-glycolic acid) (PLGA) is a popular material used to prepare nanoparticles for drug delivery. However, PLGA nanoparticles lack desirable attributes including active targeting abilities, resistance to aggregation during lyophilization, and the ability to respond to dynamic environmental stimuli. To overcome these issues, we fabricated a nanoparticle consisting of a PLGA core encapsulated within a shell of poly(N-isopropylacrylamide). Dynamic light scattering and transmission electron microscope imaging were used to characterize the nanoparticles, while an MTT assay and ELISA suggested biocompatibility in THP1 cells. Finally, a collagen type II binding assay showed successful modification of these nanoparticles with an active targeting moiety.

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