{"title":"[未分离肝素对急性肺损伤大鼠金属蛋白酶-2和-9表达的影响]。","authors":"Xu Li, Zhen Zheng, Xiao-chun Ma","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the activation of matrix metalloproteinase (MMP-2, MMP-9) in rats with acute lung injury (ALI) and the effects of unfractionated heparin (UFH) on the levels.</p><p><strong>Methods: </strong>Eighteen male Wistar rats were divided into control group, ALI group and UFH group, with 6 rats in each group by means of random number table. ALI was induced by administering a bolus injection of lipopolysaccharide (LPS) via the caudal vein at a dose of 6 mg/kg. In UFH group the rats were treated intravenously with 100 U/kg of UFH 15 minutes before the injection of LPS. In control group, the rats were treated with the same volume of normal saline. Serum levels of MMP-2 and MMP-9 were measured by enzyme linked immunosorbent assay (ELISA) at 1, 3, 6 hours via femoral vein. Six hours after the injection of reagents, the rats were sacrificed and lung tissue samples were collected for mRNA analysis of the MMP-2 and MMP-9 by real-time fluorescence quantitate reverse transcription-polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>Compared with control group, the content of MMP-2 and MMP-9 in serum of ALI group were increased, and reached the peak at 6 hours (MMP-2: 2.86±0.40 μg/L vs. 1.21±0.24 μg/L, MMP-9: 2.54±0.29 μg/L vs. 1.15±0.34 μg/L, both P<0.01); they were down-regulated in UFH group at 6 hours (MMP-2: 1.92±0.31 μg/L vs. 2.86±0.40 μg/L, MMP-9: 1.82±0.26 μg/L vs. 2.54±0.29 μg/L, both P<0.05). Compared with control group, the mRNA expressions of MMP-2 and MMP-9 in the lung tissue of ALI group were increased at 6 hours (MMP-2 mRNA: 1.88±0.09 vs. 1.00±0.10, MMP-9 mRNA: 3.15±0.47 vs. 1.00±0.17, both P<0.01); they were down-regulated in UFH group (MMP-2 mRNA: 1.26±0.14 vs. 1.88±0.09, P<0.01; MMP-9 mRNA: 2.06±0.68 vs. 3.15±0.47, P<0.05), but still above the control group (both P<0.05).</p><p><strong>Conclusions: </strong>The present study demonstrated that the level of MMP-2 and MMP-9 increased in rats with ALI. UFH could exert protective effects by inhibiting expression of MMP-2 and MMP-9 in serum and lung tissue, in both mRNA and protein expression.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 10","pages":"608-11"},"PeriodicalIF":0.0000,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effects of unfractionated heparin on expression of metalloproteinase-2 and -9 in acute lung injury rats].\",\"authors\":\"Xu Li, Zhen Zheng, Xiao-chun Ma\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To determine the activation of matrix metalloproteinase (MMP-2, MMP-9) in rats with acute lung injury (ALI) and the effects of unfractionated heparin (UFH) on the levels.</p><p><strong>Methods: </strong>Eighteen male Wistar rats were divided into control group, ALI group and UFH group, with 6 rats in each group by means of random number table. ALI was induced by administering a bolus injection of lipopolysaccharide (LPS) via the caudal vein at a dose of 6 mg/kg. In UFH group the rats were treated intravenously with 100 U/kg of UFH 15 minutes before the injection of LPS. In control group, the rats were treated with the same volume of normal saline. Serum levels of MMP-2 and MMP-9 were measured by enzyme linked immunosorbent assay (ELISA) at 1, 3, 6 hours via femoral vein. Six hours after the injection of reagents, the rats were sacrificed and lung tissue samples were collected for mRNA analysis of the MMP-2 and MMP-9 by real-time fluorescence quantitate reverse transcription-polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>Compared with control group, the content of MMP-2 and MMP-9 in serum of ALI group were increased, and reached the peak at 6 hours (MMP-2: 2.86±0.40 μg/L vs. 1.21±0.24 μg/L, MMP-9: 2.54±0.29 μg/L vs. 1.15±0.34 μg/L, both P<0.01); they were down-regulated in UFH group at 6 hours (MMP-2: 1.92±0.31 μg/L vs. 2.86±0.40 μg/L, MMP-9: 1.82±0.26 μg/L vs. 2.54±0.29 μg/L, both P<0.05). Compared with control group, the mRNA expressions of MMP-2 and MMP-9 in the lung tissue of ALI group were increased at 6 hours (MMP-2 mRNA: 1.88±0.09 vs. 1.00±0.10, MMP-9 mRNA: 3.15±0.47 vs. 1.00±0.17, both P<0.01); they were down-regulated in UFH group (MMP-2 mRNA: 1.26±0.14 vs. 1.88±0.09, P<0.01; MMP-9 mRNA: 2.06±0.68 vs. 3.15±0.47, P<0.05), but still above the control group (both P<0.05).</p><p><strong>Conclusions: </strong>The present study demonstrated that the level of MMP-2 and MMP-9 increased in rats with ALI. UFH could exert protective effects by inhibiting expression of MMP-2 and MMP-9 in serum and lung tissue, in both mRNA and protein expression.</p>\",\"PeriodicalId\":23992,\"journal\":{\"name\":\"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue\",\"volume\":\"24 10\",\"pages\":\"608-11\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:探讨急性肺损伤(ALI)大鼠基质金属蛋白酶(MMP-2、MMP-9)的活性及未分离肝素(UFH)对其水平的影响。方法:18只雄性Wistar大鼠按随机数字表法分为对照组、ALI组和UFH组，每组6只。ALI是通过尾静脉注射脂多糖(LPS)，剂量为6mg /kg诱导的。UFH组大鼠在注射LPS前15分钟静脉注射100 U/kg UFH。对照组给予等量生理盐水治疗。采用酶联免疫吸附法(ELISA)测定经股静脉1、3、6小时血清中MMP-2和MMP-9的水平。注射试剂6 h后，处死大鼠，采集肺组织标本，采用实时荧光定量逆转录-聚合酶链反应(qRT-PCR)分析MMP-2和MMP-9的mRNA表达。结果:与对照组相比，ALI组血清中MMP-2和MMP-9含量升高，并在6 h达到峰值(MMP-2: 2.86±0.40 μg/L vs. 1.21±0.24 μg/L, MMP-9: 2.54±0.29 μg/L vs. 1.15±0.34 μg/L)。结论:本研究表明ALI大鼠血清中MMP-2和MMP-9水平升高。UFH可能通过抑制血清和肺组织中MMP-2和MMP-9 mRNA和蛋白的表达来发挥保护作用。
[Effects of unfractionated heparin on expression of metalloproteinase-2 and -9 in acute lung injury rats].
Objective: To determine the activation of matrix metalloproteinase (MMP-2, MMP-9) in rats with acute lung injury (ALI) and the effects of unfractionated heparin (UFH) on the levels.
Methods: Eighteen male Wistar rats were divided into control group, ALI group and UFH group, with 6 rats in each group by means of random number table. ALI was induced by administering a bolus injection of lipopolysaccharide (LPS) via the caudal vein at a dose of 6 mg/kg. In UFH group the rats were treated intravenously with 100 U/kg of UFH 15 minutes before the injection of LPS. In control group, the rats were treated with the same volume of normal saline. Serum levels of MMP-2 and MMP-9 were measured by enzyme linked immunosorbent assay (ELISA) at 1, 3, 6 hours via femoral vein. Six hours after the injection of reagents, the rats were sacrificed and lung tissue samples were collected for mRNA analysis of the MMP-2 and MMP-9 by real-time fluorescence quantitate reverse transcription-polymerase chain reaction (qRT-PCR).
Results: Compared with control group, the content of MMP-2 and MMP-9 in serum of ALI group were increased, and reached the peak at 6 hours (MMP-2: 2.86±0.40 μg/L vs. 1.21±0.24 μg/L, MMP-9: 2.54±0.29 μg/L vs. 1.15±0.34 μg/L, both P<0.01); they were down-regulated in UFH group at 6 hours (MMP-2: 1.92±0.31 μg/L vs. 2.86±0.40 μg/L, MMP-9: 1.82±0.26 μg/L vs. 2.54±0.29 μg/L, both P<0.05). Compared with control group, the mRNA expressions of MMP-2 and MMP-9 in the lung tissue of ALI group were increased at 6 hours (MMP-2 mRNA: 1.88±0.09 vs. 1.00±0.10, MMP-9 mRNA: 3.15±0.47 vs. 1.00±0.17, both P<0.01); they were down-regulated in UFH group (MMP-2 mRNA: 1.26±0.14 vs. 1.88±0.09, P<0.01; MMP-9 mRNA: 2.06±0.68 vs. 3.15±0.47, P<0.05), but still above the control group (both P<0.05).
Conclusions: The present study demonstrated that the level of MMP-2 and MMP-9 increased in rats with ALI. UFH could exert protective effects by inhibiting expression of MMP-2 and MMP-9 in serum and lung tissue, in both mRNA and protein expression.
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