不同培养条件下粘红酵母同时产超氧化物歧化酶和过氧化氢酶的研究。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-10-01 Epub Date: 2012-04-03 DOI:10.3109/10731199.2012.668910

Ayşe Ezgi Unlü, Serpil Takaç

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引用次数: 7

摘要

研究了粘红酵母同时生产超氧化物(SOD)和过氧化氢酶(CAT)的方法。研究了温度、初始培养基pH和碳源对酶活性的影响。温度和碳源对酶活性有显著影响。10°C的CAT和SOD活性最高，分别为22.6 U/mg蛋白和170 U/mg蛋白。甘油是最佳的酶活性碳源，为CAT提供113 U/mg蛋白质，为SOD提供125 U/mg蛋白质，也是本研究获得的最高活性。

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Investigation of the simultaneous production of superoxide dismutase and catalase enzymes from Rhodotorula glutinis under different culture conditions.

The simultaneous production production of superoxide (SOD) and catalase (CAT) from Rhodotorula glutinis was studied. The effects of temperature, initial medium pH, and carbon source on the enzyme activities were investigated. Temperature and carbon sources were found to have significant effects on the enzyme activities. 10°C provided the highest specific CAT and SOD activities as 22.6 U/mg protein and 170 U/mg protein, respectively. Glycerol was found to be the best carbon source for enzyme activities, providing 113 U/mg protein for CAT and 125 U/mg protein for SOD, which were also the highest activities obtained in the present study.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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审稿时长

6-12 weeks