在啮齿动物β细胞系中,氧化代谢基因对氧化应激没有反应。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-02-20 DOI:10.1155/2012/793783
Faer Morrison, Karen Johnstone, Anna Murray, Jonathan Locke, Lorna W Harries
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引用次数: 4

摘要

氧化代谢基因表达的改变已经在2型糖尿病患者的骨骼肌中被描述。胰腺细胞含有低水平的抗氧化酶,特别容易受到氧化应激的影响。在这项研究中,我们探讨了高血糖诱导的氧化应激对啮齿动物β细胞系氧化代谢基因的影响。我们将INS-1啮齿动物β细胞暴露于低(5.6 mmol/L)、环境(11 mmol/L)和高(28 mmol/L)葡萄糖条件下48小时。使用荧光探针二氢膦丹明123测量氧化应激的增加。然后,我们通过实时PCR测量了一组90个氧化代谢基因的表达水平。48 h后,INS-1细胞的活性氧(ROS)生成明显升高(P < 0.05)。TLDA分析显示,在高血糖条件下,90个基因中有16个基因表达显著上调(P < 0.05),尽管这些表达差异并不反映ROS的差异。我们的结论是,虽然血糖改变可能影响一些氧化代谢基因的表达,但这种影响可能不是由ROS产生增加介导的。先前在人类糖尿病骨骼肌中观察到的氧化代谢基因表达的改变似乎并没有反映在啮齿动物的胰腺细胞中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oxidative metabolism genes are not responsive to oxidative stress in rodent Beta cell lines.

Oxidative metabolism genes are not responsive to oxidative stress in rodent Beta cell lines.

Oxidative metabolism genes are not responsive to oxidative stress in rodent Beta cell lines.

Altered expression of oxidative metabolism genes has been described in the skeletal muscle of individuals with type 2 diabetes. Pancreatic beta cells contain low levels of antioxidant enzymes and are particularly susceptible to oxidative stress. In this study, we explored the effect of hyperglycemia-induced oxidative stress on a panel of oxidative metabolism genes in a rodent beta cell line. We exposed INS-1 rodent beta cells to low (5.6 mmol/L), ambient (11 mmol/L), and high (28 mmol/L) glucose conditions for 48 hours. Increases in oxidative stress were measured using the fluorescent probe dihydrorhodamine 123. We then measured the expression levels of a panel of 90 oxidative metabolism genes by real-time PCR. Elevated reactive oxygen species (ROS) production was evident in INS-1 cells after 48 hours (P < 0.05). TLDA analysis revealed a significant (P < 0.05) upregulation of 16 of the 90 genes under hyperglycemic conditions, although these expression differences did not reflect differences in ROS. We conclude that although altered glycemia may influence the expression of some oxidative metabolism genes, this effect is probably not mediated by increased ROS production. The alterations to the expression of oxidative metabolism genes previously observed in human diabetic skeletal muscle do not appear to be mirrored in rodent pancreatic beta cells.

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来源期刊
Experimental Diabetes Research
Experimental Diabetes Research 医学-内分泌学与代谢
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