Adam Pym , John G.M. Mina , Bartlomiej J. Troczka , Angela Hayward , Eve Daum , Jan Elias , Russell Slater , John Vontas , Chris Bass , Christoph T. Zimmer
{"title":"烟粉虱细胞色素p450 cyp6cm2的单点突变导致对新烟碱类的抗性增强","authors":"Adam Pym , John G.M. Mina , Bartlomiej J. Troczka , Angela Hayward , Eve Daum , Jan Elias , Russell Slater , John Vontas , Chris Bass , Christoph T. Zimmer","doi":"10.1016/j.ibmb.2023.103934","DOIUrl":null,"url":null,"abstract":"<div><p>The tobacco whitefly, <em>Bemisia tabaci</em>, is a polyphagous crop pest which causes high levels of economic damage across the globe. Insecticides are often required for the effective control of this species, among which the neonicotinoid class have been particularly widely used. Deciphering the mechanisms responsible for resistance to these chemicals is therefore critical to maintain control of <em>B. tabaci</em> and limit the damage it causes. An important mechanism of resistance to neonicotinoids in <em>B. tabaci</em> is the overexpression of the cytochrome P450 gene <em>CYP6CM1</em> which leads to the enhanced detoxification of several neonicotinoids. In this study we show that qualitative changes in this P450 dramatically alter its metabolic capacity to detoxify neonicotinoids. <em>CYP6CM1</em> was significantly over-expressed in two strains of <em>B. tabaci</em> which displayed differing levels of resistance to the neonicotinoids imidacloprid and thiamethoxam. Sequencing of the <em>CYP6CM1</em> coding sequence from these strains revealed four different alleles encoding isoforms carrying several amino acid changes. Expression of these alleles <em>in vitro</em> and <em>in vivo</em> provided compelling evidence that a mutation (A387G), present in two of the <em>CYP6CM1</em> alleles, results in enhanced resistance to several neonicotinoids. These data demonstrate the importance of both qualitative and quantitative changes in genes encoding detoxification enzymes in the evolution of insecticide resistance and have applied implications for resistance monitoring programs.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A single point mutation in the Bemisia tabaci cytochrome-P450 CYP6CM1 causes enhanced resistance to neonicotinoids\",\"authors\":\"Adam Pym , John G.M. Mina , Bartlomiej J. Troczka , Angela Hayward , Eve Daum , Jan Elias , Russell Slater , John Vontas , Chris Bass , Christoph T. Zimmer\",\"doi\":\"10.1016/j.ibmb.2023.103934\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The tobacco whitefly, <em>Bemisia tabaci</em>, is a polyphagous crop pest which causes high levels of economic damage across the globe. Insecticides are often required for the effective control of this species, among which the neonicotinoid class have been particularly widely used. Deciphering the mechanisms responsible for resistance to these chemicals is therefore critical to maintain control of <em>B. tabaci</em> and limit the damage it causes. An important mechanism of resistance to neonicotinoids in <em>B. tabaci</em> is the overexpression of the cytochrome P450 gene <em>CYP6CM1</em> which leads to the enhanced detoxification of several neonicotinoids. In this study we show that qualitative changes in this P450 dramatically alter its metabolic capacity to detoxify neonicotinoids. <em>CYP6CM1</em> was significantly over-expressed in two strains of <em>B. tabaci</em> which displayed differing levels of resistance to the neonicotinoids imidacloprid and thiamethoxam. Sequencing of the <em>CYP6CM1</em> coding sequence from these strains revealed four different alleles encoding isoforms carrying several amino acid changes. Expression of these alleles <em>in vitro</em> and <em>in vivo</em> provided compelling evidence that a mutation (A387G), present in two of the <em>CYP6CM1</em> alleles, results in enhanced resistance to several neonicotinoids. These data demonstrate the importance of both qualitative and quantitative changes in genes encoding detoxification enzymes in the evolution of insecticide resistance and have applied implications for resistance monitoring programs.</p></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174823000280\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174823000280","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A single point mutation in the Bemisia tabaci cytochrome-P450 CYP6CM1 causes enhanced resistance to neonicotinoids
The tobacco whitefly, Bemisia tabaci, is a polyphagous crop pest which causes high levels of economic damage across the globe. Insecticides are often required for the effective control of this species, among which the neonicotinoid class have been particularly widely used. Deciphering the mechanisms responsible for resistance to these chemicals is therefore critical to maintain control of B. tabaci and limit the damage it causes. An important mechanism of resistance to neonicotinoids in B. tabaci is the overexpression of the cytochrome P450 gene CYP6CM1 which leads to the enhanced detoxification of several neonicotinoids. In this study we show that qualitative changes in this P450 dramatically alter its metabolic capacity to detoxify neonicotinoids. CYP6CM1 was significantly over-expressed in two strains of B. tabaci which displayed differing levels of resistance to the neonicotinoids imidacloprid and thiamethoxam. Sequencing of the CYP6CM1 coding sequence from these strains revealed four different alleles encoding isoforms carrying several amino acid changes. Expression of these alleles in vitro and in vivo provided compelling evidence that a mutation (A387G), present in two of the CYP6CM1 alleles, results in enhanced resistance to several neonicotinoids. These data demonstrate the importance of both qualitative and quantitative changes in genes encoding detoxification enzymes in the evolution of insecticide resistance and have applied implications for resistance monitoring programs.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.