André Abts, Antonino Mavaro, Jan Stindt, Patrick J Bakkes, Sabine Metzger, Arnold J M Driessen, Sander H J Smits, Lutz Schmitt
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引用次数: 58
摘要
Nisin是由几种乳杆菌菌株产生和分泌的抗菌肽，对革兰氏阳性菌具有特异性活性。在以前的研究中，nisin是通过低pH下的阳离子交换色谱纯化的，采用1 M NaCl一步洗脱。在这里，我们描述了一种优化的净化方案，使用五步NaCl洗脱去除污染物。所得nisin不含杂质，对nisin敏感乳杆菌NZ9000具有较高的杀菌活性。纯化的nisin显示出~3 nM的IC(50)，与通过一步洗脱程序获得的nisin相比，这是十倍的改进。
Easy and rapid purification of highly active nisin.
Nisin is an antimicrobial peptide produced and secreted by several L. lactis strains and is specifically active against Gram-positive bacteria. In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl. Here, we describe an optimized purification protocol using a five-step NaCl elution to remove contaminants. The obtained nisin is devoid of impurities and shows high bactericidal activity against the nisin-sensitive L. lactis strain NZ9000. Purified nisin exhibits an IC(50) of ~3 nM, which is a tenfold improvement as compared to nisin obtained via the one-step elution procedure.
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