Li Xiao, Guixue Wang, Tao Jiang, Chaojun Tang, Xue Wu, Tin Sun
{"title":"剪切应力对粘附于特定基质的内皮祖细胞数量和功能的影响。","authors":"Li Xiao, Guixue Wang, Tao Jiang, Chaojun Tang, Xue Wu, Tin Sun","doi":"10.5301/JABB.2011.6475","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.</p><p><strong>Methods: </strong>In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.</p><p><strong>Results: </strong>The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.</p><p><strong>Conclusions: </strong>CD133 antibody has the potential application for EPCs capture.</p>","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":"9 3","pages":"193-8"},"PeriodicalIF":0.0000,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5301/JABB.2011.6475","citationCount":"7","resultStr":"{\"title\":\"Effects of shear stress on the number and function of endothelial progenitor cells adhered to specific matrices.\",\"authors\":\"Li Xiao, Guixue Wang, Tao Jiang, Chaojun Tang, Xue Wu, Tin Sun\",\"doi\":\"10.5301/JABB.2011.6475\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.</p><p><strong>Methods: </strong>In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.</p><p><strong>Results: </strong>The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.</p><p><strong>Conclusions: </strong>CD133 antibody has the potential application for EPCs capture.</p>\",\"PeriodicalId\":51074,\"journal\":{\"name\":\"Journal of Applied Biomaterials & Biomechanics\",\"volume\":\"9 3\",\"pages\":\"193-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.5301/JABB.2011.6475\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Biomaterials & Biomechanics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5301/JABB.2011.6475\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biomaterials & Biomechanics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5301/JABB.2011.6475","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of shear stress on the number and function of endothelial progenitor cells adhered to specific matrices.
Purpose: The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.
Methods: In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.
Results: The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.
Conclusions: CD133 antibody has the potential application for EPCs capture.
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