一种简单、快速的纯化人血浆中触珠蛋白的方法。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-04-01 DOI:10.3109/10731199.2010.509705

Lijing Sun, Yongdong Huang, Yan Zhang, Qingqiang Meng, Jian Luo, Bei Fan, Guanghui Ma, Zhiguo Su

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引用次数: 5

摘要

人血浆ⅳ组分是冷乙醇法生产人血清白蛋白过程中的中间沉淀。位于该部分的触珠蛋白可以纯化用于各种应用。采用聚乙二醇(PEG)沉淀-离子交换色谱(IEC)相结合的工艺纯化了珠蛋白，可有效地将珠蛋白的纯度从16.6%提高到95%。新工艺的回收率为58.2%，而传统亲和色谱法的回收率为30.3%。此外，175 mg触珠蛋白的规模化生产表明，该方法简单、快速、低成本。

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A simple and rapid procedure for purification of haptoglobin from human plasma fraction IV.

Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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审稿时长

6-12 weeks