新型席夫碱聚合物上β-半乳糖苷酶的固定化。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-08-01 Epub Date: 2011-02-16 DOI:10.3109/10731199.2011.555837

Elif Aynacı, Nurşen Sarı, Hayrettin Tümtürk

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引用次数: 14

摘要

我们开发了一种固定β-半乳糖苷酶作为模型酶的策略，采用具有希夫碱的聚合物载体，由(氨基甲基)聚苯乙烯和2-苯吲哚-3-甲醛缩合而成。通过共价键将β-半乳糖苷酶固定在新的聚合物载体上。研究了温度、pH、可重复使用性和储存容量对游离和固定化β-半乳糖苷酶的影响。结果表明，含席夫碱的(氨基甲基)聚苯乙烯最适合于β-半乳糖苷酶的固定。这些新型载体具有储存能力强、可重复使用等优点，可用于β-半乳糖苷酶的固定化。

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Immobilization of β-galactosidase on novel polymers having Schiff bases.

We have developed a strategy to immobilize β-galactosidase as a model enzyme by using polymeric supports having Schiff bases, which were prepared from (aminomethyl)polystyrene and 2-phenlyindole-3-carboxaldehyde by condensation. β-galactosidase was immobilized onto the new polymer supports via covalent bonds. The influence of temperature, pH, reusability, and storage capacity on the free and immobilized β-galactosidase was investigated. Our results indicate that the (aminomethyl)polystyrene with Schiff bases is most suitable for the immobilization of β-galactosidase. These kinds of new supports can be used for the immobilization of β-galactosidase due to their strong storage capacity and reusability.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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6-12 weeks