Al2O3上游离和固定化念珠菌脂肪酶的生化特性比较研究。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-08-01 Epub Date: 2010-11-30 DOI:10.3109/10731199.2010.533125

Yeşim Yeşiloğlu, Latifşah Şit

{"title":"Al2O3上游离和固定化念珠菌脂肪酶的生化特性比较研究。","authors":"Yeşim Yeşiloğlu, Latifşah Şit","doi":"10.3109/10731199.2010.533125","DOIUrl":null,"url":null,"abstract":"Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":"39 4","pages":"247-51"},"PeriodicalIF":0.0000,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.533125","citationCount":"6","resultStr":"{\"title\":\"Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.\",\"authors\":\"Yeşim Yeşiloğlu, Latifşah Şit\",\"doi\":\"10.3109/10731199.2010.533125\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.\",\"PeriodicalId\":8413,\"journal\":{\"name\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"volume\":\"39 4\",\"pages\":\"247-51\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/10731199.2010.533125\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10731199.2010.533125\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2010/11/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial cells, blood substitutes, and immobilization biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10731199.2010.533125","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2010/11/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 6

摘要

采用Al(2)O(3)物理吸附法固定化了念珠菌(Candida rugosa, CRL)脂肪酶。在30 min内固定化产率可达95%以上。并对游离酶和固定化酶的性质进行了比较。通过固定化酶使最佳pH由7改变为8.5。游离酶和固定化酶的活性分别在35℃和40℃达到最高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.

Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

自引率

0.00%

发文量

审稿时长

6-12 weeks