{"title":"嗜酸Euryarchaeon热原体Cdc6同源物的鉴定。","authors":"Gyri Teien Haugland, Marte Innselset, Dominique Madern, Nils-Kåre Birkeland","doi":"10.2174/1874091X00802010129","DOIUrl":null,"url":null,"abstract":"<p><p>Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.</p>","PeriodicalId":515405,"journal":{"name":"The Open Biochemistry Journal","volume":"2 ","pages":"129-34"},"PeriodicalIF":0.0000,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0a/b5/TOBIOCJ-2-129.PMC2627522.pdf","citationCount":"5","resultStr":"{\"title\":\"Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.\",\"authors\":\"Gyri Teien Haugland, Marte Innselset, Dominique Madern, Nils-Kåre Birkeland\",\"doi\":\"10.2174/1874091X00802010129\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.</p>\",\"PeriodicalId\":515405,\"journal\":{\"name\":\"The Open Biochemistry Journal\",\"volume\":\"2 \",\"pages\":\"129-34\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0a/b5/TOBIOCJ-2-129.PMC2627522.pdf\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Open Biochemistry Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874091X00802010129\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2008/9/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Biochemistry Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874091X00802010129","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2008/9/27 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.
Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.
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