玉米(Zea mays)品种青贮中细菌群落描述方法的探索。

Environmental biosafety research Pub Date : 2008-01-01 Epub Date: 2008-04-03 DOI:10.1051/ebr:2007047
Lorenzo Brusetti, Sara Borin, Aurora Rizzi, Diego Mora, Claudia Sorlini, Daniele Daffonchio
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引用次数: 11

摘要

采用4个不同的玉米品种(3个常规玉米品种和1个转基因玉米品种)制备青贮饲料,研究了不同的细菌群落结构和多样性评价技术。苔原,事件Bt-176)。植物在温室中种植,生长30天后收获。在发酵过程中连续收集青贮样品,并通过各种基于dna的指纹技术分析细菌计数。可培养菌总数、孢子形成菌数、嗜中温和嗜热乳酸菌(LAB)的数量在不同品种之间相似。通过基因间转录间隔(ITS) PCR和16S rRNA基因测序对388株LAB菌株的物种组成进行进一步分析,未发现品种间的差异。相比之下,针对整个细菌群落的分子指纹分析方法,如自动核糖体基因间间隔分析(ARISA)和16S rRNA基因长度异质性pcr (LH-PCR),表明不同的玉米青贮批次或品种承载不同的细菌群落。因此,ARISA和LH-PCR指纹技术提供了一种快速、灵敏的方法来比较细菌群落,并检测青贮细菌群落的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploration of methods used to describe bacterial communities in silage of maize (Zea mays) cultivars.

Different techniques to assess bacterial community structure and diversity were evaluated in silages prepared with four different maize cultivars, three conventional and one transgenic (cv. Tundra, event Bt-176). Plants were cultivated in the greenhouse and harvested after 30 days of growth. Silage samples were collected at successive times during fermentation and analyzed for bacterial counts and by various DNA-based fingerprinting techniques. Bacterial counts were similar between cultivars for the total culturable bacteria, sporeforming, and mesophilic and thermophilic lactic acid bacteria (LAB). Further analysis of the species composition of 388 LAB strains by intergenic transcribed spacer (ITS) PCR followed by sequencing of 16S rRNA gene did not reveal differences between cultivars. In contrast, molecular fingerprinting methods targeting whole bacterial communities, such as automated ribosomal intergenic spacers analysis (ARISA) and 16S rRNA gene length heterogeneity-PCR (LH-PCR), indicated that different maize silage batches or cultivars hosted different bacterial communities. Thus, ARISA and LH-PCR fingerprinting techniques offer a fast and sensitive method to compare bacterial communities, and to detect differences in silage bacterial communities.

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