Xin Hu , Shengxian Yu , Guang Yang , Wei Long , Teng Guo , Jianwen Tian , Meiying Liu , Xiancai Li , Xiaoyong Zhang , Yen Wei
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引用次数: 7
摘要
壳聚糖是一种丰富的天然多糖,含有大量的氨基和羟基。它具有低毒、可生物降解和低成本等特点,在生物医学领域具有很大的应用潜力。本文设计并合成了一种新型的壳聚糖基荧光共聚物(WS-CS- tpa),通过亲核取代六氯环三磷腈(HCCP)、水溶性壳聚糖(WS-CS)和聚集诱导发射(AIE)氟化合物(AIEgen)三苯胺衍生物(TPA-NH2)。超声处理下,室温下0.7 g HCCP可偶联1.16 g TPA-NH2和1.1 g WS-CS。所得共聚物具有两亲性,可组装成尺寸约为100 nm的纳米颗粒。自组装后,TPA-NH2聚集在核心,在水介质中表现出极好的AIE特征,具有强烈的绿色荧光发射。另一方面,将亲水性WS-CS包覆在纳米颗粒表面,使其具有较高的水分散性。初步的生物学实验结果表明,WS-CS-TPA可被细胞内化,具有较低的细胞毒性,在生物成像和细胞内给药方面具有很大的潜力。
Fabrication of chitosan based luminescent nanoprobe with aggregation-induced emission feature through ultrasonic treatment
Chitosan is an abundant natural polysaccharide that contains a lot of amino and hydroxyl groups. It possesses great potential for biomedical applications owing to its low toxicity, biodegradability and low cost. Herein, a novel chitosan-based fluorescent copolymer (WS-CS-TPA) was designed and synthesized via nucleophilic substitution of hexachlorocyclotriphosphazene (HCCP), water-soluble chitosan (WS-CS) and an aggregation-induced emission (AIE) fluorogen (AIEgen) triphenylamine derivative (TPA-NH2). Under ultrasonic treatment, 1.16 g TPA-NH2 and 1.1 g WS-CS can be conjugated by 0.7 g HCCP at room temperature. The obtained copolymer shows amphiphilic property and could assemble into nanoparticles with size about 100 nm. After self-assembly, TPA-NH2 was aggregated in the core, thus exhibiting superb AIE feature with intense green fluorescence emission in aqueous media. On the other hand, hydrophilic WS-CS was coated on the surface of nanoparticles and endowed their high water dispersibility. Results from preliminary biological assays suggested that WS-CS-TPA can be internalized by cells and exhibits low cytotoxicity, suggesting their great potential for biological imaging and intracellular drug delivery.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.