[高效液相色谱-蒸发光散射检测法测定苦杏仁苷含量]。

Zhao-hui Wu, Wen-wei You, Feng He
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引用次数: 0

摘要

采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定苦杏仁苷的含量。色谱柱为Hypersil-ODS (4.6 mm × 250 mm, 5微米),柱温为25℃,流动相为甲醇-水(70:30),流速为0.5 ml/min。采用蒸发光散射检测仪,漂移管温度设置为98℃,气体流速为3.2 L/min。苦杏仁苷含量在1.0 ~ 15.1 μ g范围内呈良好的线性关系(r=0.999 9),平均加样回收率为99.0%,RSD为2.9% (n=5)。该方法简便、快速,结果准确、重现性好,可用于苦杏仁药材的质量控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Determination of amygdalin content in Semen Armeniacae Amarum by high-performance liquid chromatography with evaporative light-scattering detection].

High-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) was employed for determination of amygdalin content in Semen Armeniacae Amarum. The detection was performed with the column of Hypersil-ODS (4.6 mm x 250 mm, 5 microm) and column temperature of 25 degrees C. The mobile phase was methanol-water (70:30) with flow rate of 0.5 ml/min. Evaporative light-scattering detector was used and the drift tube temperature was set at 98 degrees Celsius with the gas flow rate of 3.2 L/min. A standard curve was generated, which was linear in the range of 1.0-15.1 microg for amygdalin content (r=0.999 9). The average recovery of amygdalin was 99.0% with RSD of 2.9% (n=5). Besides simplicity and rapidness, the method yields accurate and reproducible results and can therefore be used in the quality control of Semen Armeniacae Amarum.

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