Ji-jie Hu, Dan Jin, Da-ping Quan, Shi-zhen Zhong, Jiong-hao Chen, Kuan-Hai Wei, Jie Zhao, Guo-xian Pei
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引用次数: 0
摘要
目的:以聚D, l -乳酸-羟基乙酸酯(PLGA)、骨形态发生蛋白(BMP)和骨髓源性干细胞(BMSCs)构建新型组织工程骨,并观察其修复节段性骨缺损的效果。方法:在新西兰大白兔右桡骨处诱导15 mm骨缺损,将模型随机分为3组,分别植入含有5 mg BMP和约1 × 10(6)个BMSCs的PLGA构建的组织工程骨移植物(实验组)、含有约1 × 10(6)个BMSCs的PLGA移植物(对照组)和纯PLGA移植物(空白对照组)。采用x线片评价骨缺损植入后的成骨情况,观察术后4、8、12周骨缺损组织的组织学变化,并通过图像分析测量新骨形成情况。结果:实验组骨缺损在植入12周后完全修复,3组中效果最好。空白对照组在12周时仅用纤维组织和结缔组织填充骨缺损。结论:利用PLGA、BMP和BMSCs构建的组织工程骨具有良好的骨缺损修复能力。
[Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein in rabbits].
Objective: To construct a new tissue-engineered bone with poly (D, L-lactide-co-glycolide) (PLGA), bone morphogenetic protein (BMP) and bone marrow-derived stem cells (BMSCs) and observe its effect in repairing segmental bone defects.
Methods: A 15-mm bone defect in the right radius was induced in New Zealand white rabbits, and the models were randomized into three groups to receive implantation of the tissue-engineered bone grafts constructed with PLGA carrying 5 mg BMP and about 1 x 10(6) BMSCs (experimental group), grafts of PLGA with about 1 x 10(6) BMSCs (control group), or grafts of exclusive PLGA (blank control group), respectively. The osteogenesis in the bone defect after the implantation on was evaluated X-ray films, and the histological changes of the tissues sampled from the bone defect 4, 8, and 12 weeks after operation were observed and new bone formation was measured by image analysis.
Results: The bone defect was completely repaired in the experimental group 12 weeks after the implantation, showing the best results among the 3 groups. The bone defects in the blank control group was filled with only fibrous and connective tissues at 12 weeks.
Conclusion: This tissue-engineered bone constructed with PLGA, BMP and BMSCs possesses good ability in repairing segmental bone defect.