[局部注射α, β -亚甲基ATP诱导大鼠初级传入纤维兴奋]。

Qi Zhang, Yan Zhao, Yu-ling Tian, Yuan Guo, Dong-yuan Cao, Hui-sheng Wang
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引用次数: 0

摘要

目的:观察P2X激动剂α, β -亚甲基ATP (alphabetamae -ATP)对大鼠背侧皮肤初级传入纤维的兴奋和致敏作用。方法:采用单纤维电生理记录法,对T(9)-T(13)脊神经背侧皮支分离的神经丝进行单纤维电生理记录,观察α - atp(100微mol/L, 10微L)注射到皮肤感受野对大鼠初级感觉传入单元机械阈值和自发放电的影响。结果:α β - atp注射前Adelta和C纤维的平均力学阈值分别为0.384+/-0.018和0.943+/-0.102 mN,注射后分别降至0.304+/-0.013和0.659+/-0.071 mN (P0.05)。接受野注射α - atp(10微l)诱发7.7%的Abeta纤维、66.7%的Adelta纤维和75.0%的C纤维自发放电,且α - atp诱发Adelta纤维和C纤维自发放电的比例显著高于Abeta纤维(P0.05)。在整个实验过程中均可观察到α - atp对大鼠阿德尔塔和C初级传入端放电速率的兴奋作用。结论:外周应用ATP类似物alphabetam -ATP可刺激大鼠背侧皮肤初级传入纤维的Adelta和C纤维亚群,但对β纤维无刺激作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Local injection of alpha, beta-methylene ATP induces excitation of primary afferent fibers in rats].

Objective: To observe the excitation and sensitization of dorsal cutaneous primary afferent fibers induced by P2X agonist alpha, beta-methylene ATP (alphabetame-ATP) in rats.

Methods: By means of single fiber electrophysiological recordings on the nerve filaments isolated from the dorsal cutaneous branches of the T(9)-T(13) spinal nerves, the effects of alphabetame-ATP (100 micromol/L, 10 microl) injection into the cutaneous receptive field on the mechanical threshold and spontaneous discharge of rat primary sensory afferent units were observed.

Results: The mean mechanical threshold of Adelta and C fibers prior to alphabetame-ATP injection were 0.384+/-0.018 and 0.943+/-0.102 mN, and lowered to 0.304+/-0.013 and 0.659+/-0.071 mN after the injection, respectively (P<0.05, P<0.01). The mechanical threshold of the Abeta fibers before alphabetame-ATP injection was 0.301+/-0.019 mN, and slightly lowered to 0.288+/-0.018 mN after the injection (P>0.05). Injection of alphabetame-ATP (10 microl) into the receptive fields evoked spontaneous discharge in 7.7% of the Abeta fibers, 66.7% of Adelta fibers and 75.0% of C fibres, respectively, and the proportions of Adelta and C fibers with spontaneous discharge evoked by alphabetame-ATP were significantly greater than that of Abeta fibers (P<0.05). In the control experiments, injection of saline did not significantly affect spontaneous discharge or excite the nerve fibers. The mean discharge frequency of Adelta and C fibers increased from 0.73+/-0.24 and 0.54+/-0.21 impulses/min before injection to 3.05+/-0.65 and 8.53+/-2.04 impulses/min during the injection, with subsequent reduction to 2.40+/-0.60 and 6.68+/-1.68 impulses/min in the following 5 min (P<0.05). In contrast to Adelta and C fibers, Abeta fibers exhibited no significant changes in the mean discharge frequency in response to alphabetame-ATP (0.23+/-0.09 impulses/min before injection, 0.28+/-0.09 impulses/min during injection and 0.22+/-0.14 impulses/min after injection, P>0.05). The excitatory effects of alphabetame-ATP on the discharge rate in Adelta and C primary afferent terminal could be observed in the entire course of experiment.

Conclusion: Peripheral application of alphabetame-ATP, an ATP analogue, excites and sensitizes a subpopulation of Adelta and C fibers but not Abeta fibers of rat dorsal cutaneous primary afferent fibers.

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