多种染料掺杂核壳二氧化硅纳米颗粒在淋巴细胞和髓细胞中的摄取和细胞内运输研究。

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2021-03-08 eCollection Date: 2021-01-01 DOI:10.2147/NSA.S290867
Federica Sola, Barbara Canonico, Mariele Montanari, Angela Volpe, Chiara Barattini, Chiara Pellegrino, Erica Cesarini, Michele Guescini, Michela Battistelli, Claudio Ortolani, Alfredo Ventola, Stefano Papa
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引用次数: 7

摘要

由于大多数具有生物活性的大分子都是天然的纳米结构,在相同的生物分子尺度下运作,增强了与细胞成分的相互作用。纳米技术,特别是生物医学和制药领域值得注意的努力,推动了大量关于纳米材料生物效应的研究。此外,确定纳米材料的特定物理化学性质对于评估和设计新的安全有效的治疗方法和诊断工具至关重要。在这项体外研究中,我们报告了荧光二氧化硅纳米颗粒(NPs)的物理化学特征,与生物模型(U937和PBMC细胞)相互作用,描述了特定触发的生物反应。方法:以流式细胞术和共聚焦分析为主要方法平台。然而,采用TEM, NTA, DLS和化学方法合成NPs。结果:本研究中使用的NTB700纳米粒子是荧光核壳二氧化硅纳米粒子,通过胶束辅助方法合成,其中荧光能量转移过程(称为FRET)以高效率发生。通过流式细胞术和共聚焦显微镜,我们观察到NTB700 NP摄取似乎是一个快速的、浓度、能量和细胞类型依赖的过程,没有引起显著的细胞毒性作用。我们没有观察到内化的首选途径,尽管它们的大小和可能的聚集状态可能影响它们的挤压。在这一分析水平上,我们的研究集中在溶酶体和线粒体途径上,强调两者都参与NP共定位。尽管主要的线粒体定位,在分析的时间过程中,NPs并没有诱导细胞内ROS(已知的凋亡诱导剂)的显著增加。最后,淋巴细胞和髓细胞都能够释放NPs,这对它们的生物安全性至关重要。讨论:这些数据表明,通过将成像和局部治疗应用结合在一个独特的工具中,NTB700 NPs是未来多功能系统开发的一个有前途的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells.

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells.

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells.

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells.

Introduction: Since most biologically active macromolecules are natural nanostructures, operating in the same scale of biomolecules gives the great advantage to enhance the interaction with cellular components. Noteworthy efforts in nanotechnology, particularly in biomedical and pharmaceutical fields, have propelled a high number of studies on the biological effects of nanomaterials. Moreover, the determination of specific physicochemical properties of nanomaterials is crucial for the evaluation and design of novel safe and efficient therapeutics and diagnostic tools. In this in vitro study, we report a physicochemical characterisation of fluorescent silica nanoparticles (NPs), interacting with biological models (U937 and PBMC cells), describing the specific triggered biologic response.

Methods: Flow Cytometric and Confocal analyses are the main method platforms. However TEM, NTA, DLS, and chemical procedures to synthesize NPs were employed.

Results: NTB700 NPs, employed in this study, are fluorescent core-shell silica nanoparticles, synthesized through a micelle-assisted method, where the fluorescence energy transfer process, known as FRET, occurs at a high efficiency rate. Using flow cytometry and confocal microscopy, we observed that NTB700 NP uptake seemed to be a rapid, concentration-, energy- and cell type-dependent process, which did not induce significant cytotoxic effects. We did not observe a preferred route of internalization, although their size and the possible aggregated state could influence their extrusion. At this level of analysis, our investigation focuses on lysosome and mitochondria pathways, highlighting that both are involved in NP co-localization. Despite the main mitochondria localization, NPs did not induce a significant increase of intracellular ROS, known inductors of apoptosis, during the time course of analyses. Finally, both lymphoid and myeloid cells are able to release NPs, essential to their biosafety.

Discussion: These data allow to consider NTB700 NPs a promising platform for future development of a multifunctional system, by combining imaging and localized therapeutic applications in a unique tool.

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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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