紫柳柳中SKOR钾通道的分离及功能测定。

Yahui Chen, Xuefeng Peng, Jijie Cui, Hongxia Zhang, Jiang Jiang, Zhizhong Song
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引用次数: 0

摘要

钾离子(K+)在植物生长发育中起着关键作用。然而,森林植物K+营养的分子机制研究却很少。在植物中,SKOR基因编码向外校正的shaker型K+通道,该通道负责K+在根木质部的长距离运输。在这项研究中,我们确定了紫藤(Salix purpurea)的shaker型K+通道基因,命名为SpuSKOR,并使用膜片钳电生理系统确定其功能。SpuSKOR与杨树PtrSKOR在系统发育树上紧密聚集。定量实时PCR (qRT-PCR)分析表明,SpuSKOR主要在根中表达,在K+枯竭条件下表达减少。通过HEK293-T细胞膜片钳分析发现,当细胞膜电压达到-10 mV时,SpuSKOR通道的活性被激活,并且通道活性随着膜电压的升高而增强。记录并诱导向外电流响应外部K+浓度的降低。结果表明,SpuSKOR是紫铜中典型的电压依赖的向外整流K+通道。本研究为揭示木本植物钾离子转运和分配机制提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea

Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K+ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K+ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K+ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K+ transport and distribution in woody plants.

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来源期刊
Comparative and Functional Genomics
Comparative and Functional Genomics 生物-生化与分子生物学
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