Stéphanie Miserey-Lenkei, Katarina Trajkovic, Juan Martín D'Ambrosio, Amanda J Patel, Alenka Čopič, Pallavi Mathur, Kristine Schauer, Bruno Goud, Véronique Albanèse, Romain Gautier, Melody Subra, David Kovacs, Hélène Barelli, Bruno Antonny
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A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types
Background Information
Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS-CoV-2 virus and host proteins.
Results
We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria.
Conclusions and Significance
This library should facilitate further cellular investigations, notably by imaging techniques.
期刊介绍：
The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms.
This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.
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