Amy K. Jassen , Jeffrey M. Brown , Helen N. Panas , Gregory M. Miller , Danqing Xiao , Bertha K. Madras
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Variants of the primate vesicular monoamine transporter-2
The vesicular monoamine transporter-2 (VMAT2) sequesters monoamine neurotransmitters into vesicles and prevents neurotoxicity. Human or monkey striatum generated three VMAT2 immunoreactive proteins of ∼75 kDa, ∼52–55 kDa, and ∼45 kDa. The ∼55-kDa band is considered the unglycosylated native protein. Deglycosylation of the VMAT2 from striatum or human VMAT2 expressed in HEK293 cells yielded a ∼45-kDa, but not a 55-kDa immunoreactive band. We investigated this apparent mismatch between observed molecular size and predicted size.
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