血红素加氧酶/一氧化碳通路在门脉高压大鼠血管对去甲肾上腺素反应中的作用。

IF 2.4 4区 医学 Q3 PHARMACOLOGY & PHARMACY
M A Erario, S Gonzales, S Romay, F X Eizayaga, J L Castro, A Lemberg, M L Tomaro
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引用次数: 8

摘要

1. 门脉高压(PH),肝硬化的主要综合征,产生高动力内脏循环和充血。为了阐明血红素加氧酶在血管低反应性中的作用,我们在大鼠肝前PH模型中评估了血红素加氧酶-1 (HO-1)的活性,测量了去甲肾上腺素(NA)的体内压力反应,并研究了阻断一氧化碳(CO)和一氧化氮(NO)途径的作用。2. 部分门静脉结扎术(PPVL)引起门静脉高压症。静脉注射去甲肾上腺素。制备肝脏、脾脏和肠系膜匀浆,测定HO-1活性和表达。采用四组大鼠:(i)假手术组;(ii) PPVL组;(iii)用Zn-protoporphyrin IX (ZnPPIX)预处理的假组;(iv) ZnPPIX预处理的PPVL组。各组分别在NO合成酶抑制剂N(G)-硝基- l -精氨酸甲酯(L-NAME)治疗前后进行研究。3.对于基础压和NA的压力反应,ZnPPIX和L-NAME分别抑制CO和NO通路,使假手术大鼠和PH大鼠的平均动脉压(MAP)升高。同样,当假手术或PPVL大鼠同时使用这两种抑制剂时,观察到MAP的增加幅度更大。4. 这些结果,以及仅在PH组HO-1活性和表达的增加,使我们认为血红素加氧酶/CO途径参与了PH大鼠血管对NA的反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of heme oxygenase/carbon monoxide pathway on the vascular response to noradrenaline in portal hypertensive rats.

1. Portal hypertension (PH), a major syndrome in cirrhosis, producing hyperdynamic splanchnic circulation and hyperaemia. In order to elucidate the contribution of heme oxygenase to the vascular hyporeactivity, we assessed the activity of heme oxygenase-1 (HO-1), measured the in vivo pressure response to noradrenaline (NA) and investigated the effects of blocking the carbon monoxide (CO) and nitric oxide (NO) pathways in a prehepatic model of PH in rats. 2. Portal hypertension was induced by partial portal vein ligation (PPVL). Noradrenaline was injected intravenously. Liver, spleen and mesentery homogenates were prepared for measurement of HO-1 activity and expression. Four groups of rats were used: (i) a sham group; (ii) a PPVL group; (iii) a sham group pretreated with Zn-protoporphyrin IX (ZnPPIX); and (iv) a PPVL group pretreated with ZnPPIX. Each group was studied before and after treatment with the NO synthase inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME). 3. For basal pressures and the pressure response to NA, inhibition of CO and NO pathways by ZnPPIX and L-NAME, respectively, produced an increase in mean arterial pressure (MAP) in sham-operated and in PH rats. Similarly, when both inhibitors were used together in either sham or PPVL rats, a greater increase in MAP was observed. 4. These results, together with the increased HO-1 activity and expression only in the PH group, have led us to suggest that the heme oxygenase/CO pathway is involved in the vascular response to NA in PH rats.

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来源期刊
Clinical and Experimental Pharmacology and Physiology
Clinical and Experimental Pharmacology and Physiology PHARMACOLOGY & PHARMACY-PHYSIOLOGY
自引率
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128
期刊介绍: Clinical and Experimental Pharmacology and Physiology is an international journal founded in 1974 by Mike Rand, Austin Doyle, John Coghlan and Paul Korner. Our focus is new frontiers in physiology and pharmacology, emphasizing the translation of basic research to clinical practice. We publish original articles, invited reviews and our exciting, cutting-edge Frontiers-in-Research series’.
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