非肾切除对自发性高血压大鼠肾脏结构特性的影响。

IF 2.4 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Hiroyuki Kinuno, Fumihiro Tomoda, Tsutomu Koike, Masanobu Takata, Hiroshi Inoue
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引用次数: 8

摘要

1. 为了研究肾细胞数量减少对高血压大鼠肾脏结构特性的影响,在5周龄时对自发性高血压大鼠(SHR)和Wistar-Kyoto (WKY)大鼠进行单侧肾切除术(UNX)或假手术(SO)(每种毒株每种手术n = 9)。2. 在10-12周龄时,测定最大血管扩张和灌注肾脏的血流压(F-P)和压力-肾小球滤过率(P-GFR)的关系。然后灌注固定肾脏进行组织学分析。3.在SO组中,SHR大鼠的F-P(最小肾血管阻力,反映肾小球前和肾小球后血管的总体管腔尺寸)斜率大于WKY大鼠。SHR大鼠的P-GFR(肾小球前与肾小球后血管阻力比)开始滤过的阈压高于WKY大鼠,但P-GFR(肾小球滤过能力)的斜率在两种大鼠之间没有差异。这些结果表明,尽管肾小球滤过能力正常,SHR肾脏的血管狭窄,尤其是肾小球前阻力血管。4. 在UNX动物中,得到以下结果:(i)两种菌株的F-P斜率均不受影响;(ii) WKY大鼠P-GFR起始滤过压力不变,SHR大鼠降低;(iii) WKY大鼠P-GFR斜率升高,SHR大鼠无代偿性适应性升高;(iv)组织学上,两株小鼠小叶间动脉管腔截面积和肾小球丛面积均有小幅增加。然而,血管管腔的增加在SHR大鼠中更为明显,而肾小球的扩大在WKY大鼠中更为明显。5. 这些结果表明,UNX减轻肾小球前阻力血管的血管狭窄,肾小球对UNX的结构适应(即滤过能力增加和肾小球增大)在SHR中受损。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of uninephrectomy on renal structural properties in spontaneously hypertensive rats.

1. To investigate effects of a reduction in nephron numbers on renal structural properties in hypertension, either unilateral nephrectomy (UNX) or sham operation (SO) was performed at 5 weeks of age in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats (n = 9 for each operation for each strain). 2. At 10-12 weeks of age, flow-pressure (F-P) and pressure-glomerular filtration rate (P-GFR) relationships were determined for maximally vasodilated, perfused kidneys. Kidneys were then perfusion fixed for histological analysis. 3. In the SO groups, the slope of F-P (minimal renal vascular resistance, reflecting overall luminal dimensions of pre- and post-glomerular vasculature) was greater in SHR than in WKY rats. The threshold pressure for beginning filtration at P-GFR (preglomerular to post-glomerular vascular resistance ratio) was higher in SHR than in WKY rats, but the slope of P-GFR (glomerular filtration capacity) did not differ between the two strains. These results suggest that vascular narrowing occurred, especially in the preglomerular resistance vessels in the kidneys of SHR, although glomerular filtration capacity was normal. 4. In UNX animals, the following results were obtained: (i) the slope of F-P was not affected in either strain; (ii) the pressure for beginning filtration at P-GFR was unchanged in WKY rats, but was decreased in SHR; (iii) the slope of P-GFR increased in WKY rats, but a compensatory adaptive increase was missing in SHR; and (iv) histologically, small increases in the luminal cross-sectional area of interlobular arteries and glomerular tuft area were observed in both strains. However, the increase in vascular lumen was more pronounced in SHR, whereas glomerular enlargement was greater in WKY rats. 5. These results suggested that UNX attenuates vascular narrowing of the preglomerular resistance vessels and glomerular structural adaptations to UNX (i.e. increased filtering capacity and glomerular enlargement) are impaired in SHR.

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来源期刊
Clinical and Experimental Pharmacology and Physiology
Clinical and Experimental Pharmacology and Physiology PHARMACOLOGY & PHARMACY-PHYSIOLOGY
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128
期刊介绍: Clinical and Experimental Pharmacology and Physiology is an international journal founded in 1974 by Mike Rand, Austin Doyle, John Coghlan and Paul Korner. Our focus is new frontiers in physiology and pharmacology, emphasizing the translation of basic research to clinical practice. We publish original articles, invited reviews and our exciting, cutting-edge Frontiers-in-Research series’.
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