Haim Shirin, Vladimir Kravtsov, Mark Shahmurov, Paulina Petchenko, Mona Boaz, Steven F Moss, Yona Avni, Ilana Avinoach
{"title":"p27kip1的表达与胃MALT淋巴瘤的分级呈负相关。","authors":"Haim Shirin, Vladimir Kravtsov, Mark Shahmurov, Paulina Petchenko, Mona Boaz, Steven F Moss, Yona Avni, Ilana Avinoach","doi":"10.1385/IJGC:35:1:025","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Decreased or lost expression of the cyclin-dependent kinase inhibitor p27kip1 protein has been found to be a poor prognostic factor in many cancers, including gastric cancer.</p><p><strong>Aim: </strong>To evaluate p27kip1 expression in gastric mucosa-associated lymphoid tissue (MALT) and gastric B-cell lymphoma.</p><p><strong>Methods: </strong>Fifty-two cases of gastric lymphoma, mean age 68.7 yr (range 23-90 yr), 11 of chronic Helicobacter pylori-associated gastritis, and 5 of normal gastric mucosa were studied. Patients were classified into two groups. Stage IE gastric lymphomas were defined as local gastric lymphoma of MALT and more advanced stages as advanced gastric lymphoma. Twenty-three patients diagnosed as stage IE, 13 of these were low-grade and 10 diffuse large B-cell lymphoma (DLBL). Twenty-nine patients were at stage IIE or above, 18 with low-grade and 11 with DLBL. Serial sections were evaluated by immunohistochemistry after staining with antibodies against p27/Kip1 and Ki-67.</p><p><strong>Results: </strong>The proliferative index was higher in gastric DLBL than in low-grade MALT lymphomas, 57.1+/-31.2 vs 17.3+/-20.6 (p=0.0001). The mean p27kip1 expression score for high-grade patients was significantly lower compared with that of low-grade patients, 0.5+/-0.4 and 1.6+/-0.8, respectively (p=0.001). Comparative evaluation of p27kip1 expression in malignant lymphoid cells revealed that B cells of the localized gastric DLBL patients expressed the least p27kip1, 0.36+/-0.32. This value was lower than that of malignant lymphoid cells of patients with advanced DLBL, 0.64+/-0.53, advanced low-grade MALT lymphoma, 1.59+/-0.79, and localized low-grade MALT lymphoma, 1.59+/-0.84. In the multivariate model in which all p27kip1 variables were entered, the expression of p27kip1 in malignant lymphoid cells was inversely correlated with the grade of the lymphoma irrespective of the stage of the disease (p=0.0001), and significantly predicted grade: OR:0.07, 95% CI 0.07-0.31, p=0.0001.</p><p><strong>Conclusion: </strong>p27kip1 may be a putative distinct molecular marker to differentiate between low-grade and high-grade gastric lymphoma.</p>","PeriodicalId":84927,"journal":{"name":"International journal of gastrointestinal cancer","volume":"35 1","pages":"25-32"},"PeriodicalIF":0.0000,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1385/IJGC:35:1:025","citationCount":"5","resultStr":"{\"title\":\"p27kip1 expression is inversely related to the grade of gastric MALT lymphoma.\",\"authors\":\"Haim Shirin, Vladimir Kravtsov, Mark Shahmurov, Paulina Petchenko, Mona Boaz, Steven F Moss, Yona Avni, Ilana Avinoach\",\"doi\":\"10.1385/IJGC:35:1:025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Decreased or lost expression of the cyclin-dependent kinase inhibitor p27kip1 protein has been found to be a poor prognostic factor in many cancers, including gastric cancer.</p><p><strong>Aim: </strong>To evaluate p27kip1 expression in gastric mucosa-associated lymphoid tissue (MALT) and gastric B-cell lymphoma.</p><p><strong>Methods: </strong>Fifty-two cases of gastric lymphoma, mean age 68.7 yr (range 23-90 yr), 11 of chronic Helicobacter pylori-associated gastritis, and 5 of normal gastric mucosa were studied. Patients were classified into two groups. Stage IE gastric lymphomas were defined as local gastric lymphoma of MALT and more advanced stages as advanced gastric lymphoma. Twenty-three patients diagnosed as stage IE, 13 of these were low-grade and 10 diffuse large B-cell lymphoma (DLBL). Twenty-nine patients were at stage IIE or above, 18 with low-grade and 11 with DLBL. Serial sections were evaluated by immunohistochemistry after staining with antibodies against p27/Kip1 and Ki-67.</p><p><strong>Results: </strong>The proliferative index was higher in gastric DLBL than in low-grade MALT lymphomas, 57.1+/-31.2 vs 17.3+/-20.6 (p=0.0001). The mean p27kip1 expression score for high-grade patients was significantly lower compared with that of low-grade patients, 0.5+/-0.4 and 1.6+/-0.8, respectively (p=0.001). Comparative evaluation of p27kip1 expression in malignant lymphoid cells revealed that B cells of the localized gastric DLBL patients expressed the least p27kip1, 0.36+/-0.32. This value was lower than that of malignant lymphoid cells of patients with advanced DLBL, 0.64+/-0.53, advanced low-grade MALT lymphoma, 1.59+/-0.79, and localized low-grade MALT lymphoma, 1.59+/-0.84. In the multivariate model in which all p27kip1 variables were entered, the expression of p27kip1 in malignant lymphoid cells was inversely correlated with the grade of the lymphoma irrespective of the stage of the disease (p=0.0001), and significantly predicted grade: OR:0.07, 95% CI 0.07-0.31, p=0.0001.</p><p><strong>Conclusion: </strong>p27kip1 may be a putative distinct molecular marker to differentiate between low-grade and high-grade gastric lymphoma.</p>\",\"PeriodicalId\":84927,\"journal\":{\"name\":\"International journal of gastrointestinal cancer\",\"volume\":\"35 1\",\"pages\":\"25-32\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1385/IJGC:35:1:025\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of gastrointestinal cancer\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1385/IJGC:35:1:025\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of gastrointestinal cancer","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1385/IJGC:35:1:025","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
背景:细胞周期蛋白依赖性激酶抑制剂p27kip1蛋白的表达减少或缺失已被发现是包括胃癌在内的许多癌症预后不良的因素。目的:探讨p27kip1在胃黏膜相关淋巴组织(MALT)和胃b细胞淋巴瘤中的表达。方法:52例胃淋巴瘤，平均年龄68.7岁(23 ~ 90岁)，11例慢性幽门螺杆菌相关性胃炎，5例正常胃黏膜。患者分为两组。IE期胃淋巴瘤定义为MALT的局部胃淋巴瘤，晚期定义为晚期胃淋巴瘤。23例患者诊断为IE期，其中13例为低级别，10例为弥漫性大b细胞淋巴瘤(DLBL)。IIE及以上分期29例，低分级18例，DLBL 11例。连续切片经p27/Kip1和Ki-67抗体染色后进行免疫组织化学评价。结果:胃DLBL的增殖指数高于低级别MALT淋巴瘤，分别为57.1+/-31.2 vs 17.3+/-20.6 (p=0.0001)。高级别患者的p27kip1平均表达评分显著低于低级别患者，分别为0.5+/-0.4和1.6+/-0.8 (p=0.001)。对比评价p27kip1在恶性淋巴样细胞中的表达，发现胃局部DLBL患者B细胞中p27kip1表达最少，为0.36+/-0.32。该值低于晚期DLBL患者的恶性淋巴样细胞(0.64+/-0.53)、晚期低级别MALT淋巴瘤(1.59+/-0.79)和局限性低级别MALT淋巴瘤(1.59+/-0.84)。在输入所有p27kip1变量的多变量模型中，p27kip1在恶性淋巴样细胞中的表达与淋巴瘤的分级呈负相关(p=0.0001)，无论疾病的分期如何，p27kip1的表达与淋巴瘤的分级呈负相关(p=0.0001)，并显著预测分级:OR:0.07, 95% CI 0.07-0.31, p=0.0001。结论:p27kip1可能是鉴别低级别和高级别胃淋巴瘤的分子标志物。
p27kip1 expression is inversely related to the grade of gastric MALT lymphoma.
Background: Decreased or lost expression of the cyclin-dependent kinase inhibitor p27kip1 protein has been found to be a poor prognostic factor in many cancers, including gastric cancer.
Aim: To evaluate p27kip1 expression in gastric mucosa-associated lymphoid tissue (MALT) and gastric B-cell lymphoma.
Methods: Fifty-two cases of gastric lymphoma, mean age 68.7 yr (range 23-90 yr), 11 of chronic Helicobacter pylori-associated gastritis, and 5 of normal gastric mucosa were studied. Patients were classified into two groups. Stage IE gastric lymphomas were defined as local gastric lymphoma of MALT and more advanced stages as advanced gastric lymphoma. Twenty-three patients diagnosed as stage IE, 13 of these were low-grade and 10 diffuse large B-cell lymphoma (DLBL). Twenty-nine patients were at stage IIE or above, 18 with low-grade and 11 with DLBL. Serial sections were evaluated by immunohistochemistry after staining with antibodies against p27/Kip1 and Ki-67.
Results: The proliferative index was higher in gastric DLBL than in low-grade MALT lymphomas, 57.1+/-31.2 vs 17.3+/-20.6 (p=0.0001). The mean p27kip1 expression score for high-grade patients was significantly lower compared with that of low-grade patients, 0.5+/-0.4 and 1.6+/-0.8, respectively (p=0.001). Comparative evaluation of p27kip1 expression in malignant lymphoid cells revealed that B cells of the localized gastric DLBL patients expressed the least p27kip1, 0.36+/-0.32. This value was lower than that of malignant lymphoid cells of patients with advanced DLBL, 0.64+/-0.53, advanced low-grade MALT lymphoma, 1.59+/-0.79, and localized low-grade MALT lymphoma, 1.59+/-0.84. In the multivariate model in which all p27kip1 variables were entered, the expression of p27kip1 in malignant lymphoid cells was inversely correlated with the grade of the lymphoma irrespective of the stage of the disease (p=0.0001), and significantly predicted grade: OR:0.07, 95% CI 0.07-0.31, p=0.0001.
Conclusion: p27kip1 may be a putative distinct molecular marker to differentiate between low-grade and high-grade gastric lymphoma.
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