Russell Brown , Barbara Morash , Ehud Ur , Michael Wilkinson
{"title":"rnai介导的瘦素基因表达沉默增加C6胶质母细胞瘤细胞的细胞死亡","authors":"Russell Brown , Barbara Morash , Ehud Ur , Michael Wilkinson","doi":"10.1016/j.molbrainres.2005.05.009","DOIUrl":null,"url":null,"abstract":"<div><p><span>We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (</span><em>P</em><span> < 0.05) and protein by 55% (</span><em>P</em> < 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (<em>P</em> < 0.015) and TUNEL (<em>P</em><span> < 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain.</span></p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Pages 357-360"},"PeriodicalIF":0.0000,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.009","citationCount":"21","resultStr":"{\"title\":\"RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells\",\"authors\":\"Russell Brown , Barbara Morash , Ehud Ur , Michael Wilkinson\",\"doi\":\"10.1016/j.molbrainres.2005.05.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (</span><em>P</em><span> < 0.05) and protein by 55% (</span><em>P</em> < 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (<em>P</em> < 0.015) and TUNEL (<em>P</em><span> < 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain.</span></p></div>\",\"PeriodicalId\":100932,\"journal\":{\"name\":\"Molecular Brain Research\",\"volume\":\"139 2\",\"pages\":\"Pages 357-360\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-10-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.009\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Brain Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0169328X05002317\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Brain Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0169328X05002317","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells
We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (P < 0.05) and protein by 55% (P < 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (P < 0.015) and TUNEL (P < 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain.
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